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植物研究 ›› 2003, Vol. 23 ›› Issue (3): 328-333.

• 论文 • 上一篇    下一篇

猕猴桃属植物叶绿体基因PCR-RFLP分析

李健仔, 李思光, 罗玉萍, 陈少风   

  1. 南昌大学生物科学工程系, 南昌 330047
  • 出版日期:2003-09-15 发布日期:2016-06-14
  • 通讯作者: 李思光(1962-),男,博士,教授,主要从事生物化学与分子生物学研究。
  • 作者简介:李健仔(1977-),男,南昌大学在读硕士研究生,主要从事生物化学及分子生物学研究。
  • 基金资助:
    国家自然科学基金资助项目(39960007);江西省自然科学基金资助项目(0230001)

PCR-RFLP ANALYSIS ON CHLOROPLAST DNA OF ACTINIDIA

LI Jian-Zi, LI Si-Guang, LUO Yu-Ping, CHEN Shao-Feng   

  1. Department of Biological Science and Technology, Nanchang University, Nanchang 330047
  • Online:2003-09-15 Published:2016-06-14

摘要: 分别用2个不同限制性内切酶对猕猴桃属27个种和15个栽培品种的叶绿体基因(rbcL基因和psbA基因)的PCR扩增产物进行酶切分析,共得到25个限制性位点,其中24个具有多态性。确立了该属植物的单元型分布,对该属植物的系统发育方式和部分重要种类的亲缘关系进行了探讨,拓展了可用于该属植物分子系统学研究的遗传信息。

关键词: 猕猴桃属, 叶绿体基因, 系统发育

Abstract: Twentyseven species and fifteen variations of Actinidia were investigated through PCR-RFLP analysis of two chloroplast DNA fragments, including rbcL gene and psbA gene. The two chloroplast gene fragments were digested by two restriction endonucleasea respectively, which yielded twentyfive restriction sites and twentyfour of them were polymorphic. Haplotype of Actinidia was geographically established. Pattern of phylogene in Actinidia and relations between some important species were explored. Genetic information of molecular systematics in Actinidia was expanded by above all.

Key words: Aceinidia, chloroplast DNA, phylogene