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植物研究 ›› 2006, Vol. 26 ›› Issue (2): 201-205.doi: 10.7525/j.issn.1673-5102.2006.02.018

• 论文 • 上一篇    下一篇

廊坊杨3号快繁和转基因的再生系统

吴双秀1;祖元刚2   

  1. 1.上海师范大学生物系,上海 200234 2.东北林业大学教育部森林植物生态学实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-20 发布日期:2006-03-20
  • 通讯作者: 吴双秀
  • 基金资助:
     

In-vitro regeneration of Populus langfangensis 3 for transformation and micropropagation

WU Shuang-Xiu;ZU Yuan-Gang   

  1. 1. Department of Biology, Shanghai Normal University, Shanghai 200234 2. Key Laboratory of Forest Plant Ecology of the Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: WU Shuang-Xiu
  • Supported by:
     

摘要: 对杂交杨树新品种廊坊杨3号(Populus langfangensis 3,(P. deltoides (“Shan Hai Guan”)×((P. simonii × pyramidalys)12×Ulmus pumila) 的离体叶片和茎段在附加BA、NAA、IBA和2,4-D的MS培养基上的直接和间接的器官分化、愈伤组织形成和植株再生进行了研究。叶柄和叶片最容易在叶脉处直接诱导生芽。从叶片直接诱导生芽的激素条件为1~2 mg·L-1 BA和0.5 mg·L-1 IBA,最高生芽率可达90%。2,4-D促进愈伤组织的形成。由愈伤组织诱导生芽的激素条件为0.3~0.5 mg·L-1 BA 和 0.02 mg·L-1 IBA或NAA,生芽率达76%。较好的生根条件为0.1 mg·L-1 BA和0.2~0.5 mg·L-1 IBA,生根率可达67%。以上再生条件为廊坊杨3号的转基因育种和无性快繁技术提供了可能。

关键词: 廊坊杨, 芽再生, 根再生, 愈伤组织, 植株再生

Abstract: The direct and indirect induction of organ differentiation, callus formation and plantlet regeneration from the leaf and stem explants of the hybrid Populus langfangensis 3 (P. deltoides (“Shan Hai Guan”)×((P. simonii × pyramidalys)12×Ulmus pumila) were investigated in-vitro on Murashige and Skoog (MS) medium supplemented with various concentrations of benzylaminopurine (BA), indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The petiole and leaf explant was easier for direct shoot regeneration along the veins at cutting sites. Direct shooting efficiency was related to both BA concentration and BA/IBA ratio. Efficient and rapid shooting from the leaf and stem explants was achieved on MS medium containing 1~2 mg·L-1 BA and 0.5 mg·L-1 IBA. Maximum shooting frequency reached 90%. 2,4-D promoted the callus induction of P. langfangensis 3. The shooting based on callus was obtained on MS medium supplemented with 0.3~0.5 mg·L-1 BA and 0.02 mg·L-1 IBA or NAA and the corresponding shooting frequency was 76%. Rooting based on callus succeeded on 0.1 mg·L-1 BA and 0.2~0.5 mg·L-1 IBA, yielding rooting frequency 67%. These regeneration conditions could further be used for micropropagation and transformation of P. langfangensis 3.

Key words: Populus langfangensis 3, shooting, rooting, regeneration, callus formation

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