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植物研究 ›› 2005, Vol. 25 ›› Issue (4): 453-456.doi: 10.7525/j.issn.1673-5102.2005.04.017

• 论文 • 上一篇    下一篇

沙棘不同部位总黄酮含量的测定及比较

李春英, 王微, 赵春建, 刘晓娜, 王延兵, 赵博, 祖元刚   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2005-01-17 出版日期:2005-12-15 发布日期:2016-06-14
  • 通讯作者: 祖元刚 E-mail:zygorl@vip.h.lcn
  • 作者简介:李春英(1976-),女,博士研究生,从事天然产物研究工作。
  • 基金资助:
    科技部科研条件升级改造项目(JG-2004-09);新世纪优秀人才支持计划项目(NCET-04-0316)

Comparison and determination of total flavonoids in different parts of Hippophae rhamnoides

LI Chun-Ying, WANG Wei, ZHAO Chun-Jian, LIU Xiao-Na, WANG Yan-Bing, ZHAO Bo, ZU Yuan-Gang   

  1. Key Laboratory of Forestry Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-01-17 Online:2005-12-15 Published:2016-06-14

摘要: 考察了引种于俄罗斯的大果沙棘不同部位总黄酮含量的差异。用超声提取作为沙棘总黄酮的制备方法;以芦丁为对照品,采用分光光度法作为沙棘总黄酮的测定方法。实验结果表明,沙棘叶、果肉和果皮中总黄酮含量分别为1.71%、0.76%和0.55%,叶中总黄酮含量分别是果肉和果皮的2.3倍和3.1倍,因此引种于俄罗斯的大果沙棘叶具有较大的开发价值和应用前景。

关键词: 沙棘, 总黄酮, 超声提取, 分光光度法

Abstract: The content distribution of total flavonoids in different parts of Hippophae rhamnoides L.from Russia was investigated.The sample pretreatment process involved ultrasonic extraction.The total flavonoids were determined by spectrophotometry, rutin as control sample.The experiment results showed that the contents of total flavonoids in leaf, pulp and pericarp were respectively(1.71%), (0.76%) and(0.55%), that the means content of total flavonoids in leaf is(2.3) and(3.1) times higher than that in pulp and pericarp.Thereby the leaf of Hippophae rhamnoides L.from Russia has value to be developed and used.

Key words: Hippophae rhamnoides L., total flavonoids, ultrasonic extraction, spectrophotometry