甲基紫精胁迫下转TheIF1A基因烟草的活性氧代谢

doi:10.7525/j.issn.1673-5102.2016.01.018

摘要/Abstract

摘要： 真核翻译起始因子eIF1家族基因具有一定的抗逆调节能力。前期研究表明柽柳TheIF1A基因能提高转基因植物的抗旱耐盐胁迫能力。本研究旨在对该基因的抗氧化能力进行分析，探讨TheIF1A基因是否具有抗氧化能力。组织化学染色结果显示，甲基紫精胁迫下转TheIF1A基因烟草叶片、保卫细胞、根尖积累的活性氧明显少于野生型烟草（WT），H₂O₂含量测定结果也表明转基因株系的H₂O₂含量显著低于非转基因株系。此外，甲基紫精胁迫下转TheIF1A基因烟草的CAT活性、POD活性显著高于WT株系，表明过表达TheIF1A可能通过提高保护酶活性来调节体内活性氧清除能力进而改善植株活性氧积累，提高抗氧化能力。

关键词: 刚毛柽柳, 甲基紫精胁迫, 活性氧代谢, TheIF1A

Abstract: Eukaryotic translation initiation factor eIF1 involved abiotic stress regulation in plant. Previous studies showed that overexpressed TheIF1A gene from Tamarix hispida improved drought and salt resistance of genetically modified plants. We further studied the function of TheIF1A response to methyl viologen(MV). The results of histochemical stainning showed that ROS were less accumulated in leaves, guard cells and root tips of the TheIF1A transgenic lines than WT tobacco under MV stress. The H₂O₂ content in transgenic lines were also less than WT under MV stress. Accordingly, the CAT and POD activity in transgenic lines were obviously higher than WT under MV stress. Therefore, TheIF1A could improve plant oxidative stress resistance by regulating the protecting enzymes activity to increase the ROS scavenging ability.

Key words: Tamarix hispida, methyl viologen, ROS metabolism, TheIF1A

赵玉琳1；杨桂燕1,2；于丽丽1；郭宇聪1；赵震1；高彩球1*. 甲基紫精胁迫下转TheIF1A基因烟草的活性氧代谢[J]. 植物研究, 2016, 36(1): 129-133.

ZHAO Yu-Lin1；YANG Gui-Yan1,2；YU Li-Li1；GUO Yu-Cong1；ZHAO Zhen1；GAO Cai-Qiu1*. Reactive Oxygen Metabolism of the TheIF1A Transgenic Tobacco under Methyl Viologen Stress[J]. Bulletin of Botanical Research, 2016, 36(1): 129-133.

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