小黑杨FLC基因的克隆及功能解析

doi:10.7525/j.issn.1673-5102.2015.03.007

摘要/Abstract

摘要： MADS-box家族蛋白是一类重要的调控开花的转录因子，FLC是其家族成员的编码基因之一。FLC在调节开花过程，其功能的发挥受多个途径的调控。本研究利用RT-PCR方法在小黑杨雄花芽中克隆出FLC基因的全长cDNA序列PnFLC。该基因的开放读码框为726 bp，编码241个氨基酸残基组成的分子量为27.559 kD的蛋白质，蛋白质的等电点为9.37。实时定量荧光PCR结果显示，春化能够使PnFLC在小黑杨根、茎、叶各组织中表达量下调57.9%~84%；启动子GUS染色结果表明，PnFLC启动子在分裂活跃的组织中活性较高。拟南芥的遗传转化结果显示，PnFLC可以调控AtAP1、AtSOC1和AtFT基因的表达，同时延迟拟南芥的开花时间。

关键词: 小黑杨, FLC基因, 开花调控, 春化

Abstract: MADS-box family protein is an important class of transcription factors regulating flowering. FLC is a member of MADS-box transcription factors, and the FLC gene is controlled by many pathways, and then to regulate the flowering. We cloned a FLC-homolog gene in male flower buds from Populus simonii×Populus nigra. The open reading frame (ORF) of this gene had 726 bp which encoded a protein containing 241 amino acid. The molecular weight of the predicted protein was 27.559 KD, and the pI was 9.37. By real-time quantitative PCR, vernalization could down-regulate the expression of PnFLC 57.9%-84% in the root, stem and leaves tissues of poplar. By GUS staining, the promoter of the PnFLC is highly expressed in actively dividing tissues. Furthermore, by transforming Arabopsis, PnFLC down-regulated the expression of AtAP1, AtSOC1 and AtFT, while delayed the flowering in Arabidopsis.

Key words: Populus simonii×, Populus nigra, FLC gene, flowering, vernalization

中图分类号:

孙琦；曲春浦；郑美珠；刘关君. 小黑杨FLC基因的克隆及功能解析[J]. 植物研究, 2015, 35(3): 363-369.

SUN Qi；QU Chun-Pu；ZHENG Mei-Zhu；LIU Guan-Jun. Isolation and Functional Analysis of FLC Gene from Poplar (Populus simonii×Populus nigra)[J]. Bulletin of Botanical Research, 2015, 35(3): 363-369.

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