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植物研究 ›› 2015, Vol. 35 ›› Issue (2): 240-249.doi: 10.7525/j.issn.1673-5102.2015.02.012

• 论文 • 上一篇    下一篇

番茄转录因子SlYABBY5的克隆与表达分析

    

  1. 1.重庆大学生物工程学院,重庆 400044;
    2.湖北省黄石市蔬菜科学研究所,湖北 435002
  • 出版日期:2015-03-15 发布日期:2015-05-05
  • 基金资助:
     

Cloning and Expression Analysis of a Transcription Factor SlYABBY5 in Solanum lycopersicum

    

  1. 1.Bioengineering College,Chongqing University,Chongqing 400044;
    2.Huangshi City,Hubei Academic of Vegetable Science,Hubei 435002
  • Online:2015-03-15 Published:2015-05-05
  • Supported by:
     

摘要: YABBY家族是植物中特有的一个小基因家族,具有典型N端C2C2型锌指结构域和C端螺旋环螺旋YABBY结构域。经同源序列比对分析,从番茄(Solanum Lycopersicon)中克隆了YABBY家族的一个转录因子,将其命名为SlYABBY5(GenBank登录号为AK246138)。生物信息学分析表明,SlYABBY5基因ORF长为549 bp,编码182个氨基酸残基,相对分子量为20.58 kD,等电点为8.78。实时荧光定量PCR表达模式分析表明,SlYABBY5基因可能参与果实和叶片的发育;SlYABBY5在IAA、ABA、GA3、ASA和低温胁迫下表达量下调,在高盐和叶伤胁迫下表达量上调。研究结果初步证实SlYABBY5参与番茄的生长发育以及响应非生物逆境胁迫,为SlYABBY5基因参与逆境信号途径及番茄生长发育的研究提供了理论依据。

关键词: YABBY家族, SlYABBY5, 表达模式分析, 非生物逆境胁迫

Abstract: YABBY family is a small plant-specific gene family, with a typically N-terminal C2C2-type zinc finger domain and the C-terminal helix-loop-helix YABBY domain. By homologous sequence analysis, a YABBY transcription factor was cloned from tomato (Solanum lycopersicon), and named SlYABBY5(GenBank accession number: AK246138). By bioinformatics analysis, the length of SlYABBY5 ORF is 549 bp, encoding 182 amino acid residues with relative molecular weight of 20.58 kD and an isoelectric point of 8.78. By real-time PCR analysis, SlYABBY5 gene might be involved in the development of tomato fruits and leaves. Expression of SlYABBY5 down-regulated under the treatments of IAA, ABA, GA3, ASA and low temperature stress, while up-regulated under salt stress and leaves wounding. Our results suggest that SlYABBY5 might participate in the growth and development of tomato and abiotic stress responses, and provide the basis for unrevealing the function of SlYABBY5 gene in tomato growth and development as well as stress signaling pathway.

Key words: YABBY family, SlYABBY5 gene, expression pattern analysis, abiotic stress

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