莲瓣王捧瓣与舌瓣SSH文库构建及EST序列分析

doi:10.7525/j.issn.1673-5102.2012.02.016

植物研究 ›› 2012, Vol. 32 ›› Issue (2): 222-226.doi: 10.7525/j.issn.1673-5102.2012.02.016

莲瓣王捧瓣与舌瓣SSH文库构建及EST序列分析

武芸芸¹;田璐¹;朱永平²;和凤美^1*

1.云南农业大学园林园艺学院，昆明　650201；2.云南农业大学农学与生物技术学院，昆明　650201

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-03-20 发布日期:2012-03-20
通讯作者: 和凤美
基金资助:

Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan

WU Yun-Yun;TIAN Lu;ZHU Yong-Ping;HE Feng-Mei*

1.College of Horticulture and Landscape,Yunnan Agricultural University,Kunming　650201；2.College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming　650201

Received:1900-01-01 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20
Contact: HE Feng-Mei
Supported by:

摘要/Abstract

摘要： 为了探索兰花舌瓣形成的分子机制，本研究利用消减抑制杂交技术构建了莲瓣王（Cymbidium lianpan Tang et Wang）捧瓣与舌瓣间的cDNA文库。对正向文库中随机挑选的147个阳性克隆测序，组装、拼接，找到37个唯一序列，其中包含25个单一序列和12个拼接序列。经同源性比对，这些EST分别归类于蛋白质折叠，结合、未知功能、应激反应、光合作用、转录调控、信号传导、生物合成、新陈代谢、MADS等10类。通过对MADS-box基因的系统进化树分析，可初步推测MADS-Contig1和MADS-Contig2属于A类基因中的SQUA-like亚家族基因。

关键词: 莲瓣王, 捧瓣, 舌瓣, 抑制消减杂交, MADS基因

Abstract: In order to explore the molecular mechanism of orchid labellum formation, the suppression subtractive hybridization (SSH) cDNA library was constructed with petals and labellum of Cymbidium lianpan in this study. One hundred and forty-seven randomly selected clones of positive library were sequenced, assembled and spliced, 37 unique sequences were found, containing 25 single sequences and 12 spliced sequences. After homology comparison, these ESTs were classified as protein folding, binding, unknown function, stress response, photosynthesis, transcription regulation, signal transduction, biosynthesis, metabolism, MADS and other 10 categories. Through the analysis of phylogenetic tree about MADS-box genes, we can initially speculate that MADS-Contig1 and MADS-Contig2 belong to A class SQUA-like subfamily genes.

Key words: Cymbidium lianpan, petals, labellum, suppression subtractive hybridization, MADS genes

中图分类号:

S682.1

武芸芸;田璐;朱永平;和凤美*. 莲瓣王捧瓣与舌瓣SSH文库构建及EST序列分析[J]. 植物研究, 2012, 32(2): 222-226.

WU Yun-Yun;TIAN Lu;ZHU Yong-Ping;HE Feng-Mei*. Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan[J]. Bulletin of Botanical Research, 2012, 32(2): 222-226.

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莲瓣王捧瓣与舌瓣SSH文库构建及EST序列分析

Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan

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