植物研究 ›› 2022, Vol. 42 ›› Issue (5): 886-895.doi: 10.7525/j.issn.1673-5102.2022.05.020
李赵毅1, 郝龙飞1, 刘婷岩1, 何炎红1, 张友2, 白淑兰1(
), 杨昕瑜1
收稿日期:2021-10-20
出版日期:2022-09-20
发布日期:2022-09-15
通讯作者:
白淑兰
E-mail:baishulan2004@163.com
作者简介:李赵毅(1998—),男,硕士研究生,主要从事林木菌根技术研究。
基金资助:
Zhaoyi LI1, Longfei HAO1, Tingyan LIU1, Yanhong HE1, You ZHANG2, Shulan BAI1(
), Xinyu YANG1
Received:2021-10-20
Online:2022-09-20
Published:2022-09-15
Contact:
Shulan BAI
E-mail:baishulan2004@163.com
About author:LI Zhaoyi(1998—),male,master degree candidate,mainly engaged in the research of mycorrhizal biotechnology.
Supported by:摘要:
为了解菌根化处理的灌木铁线莲(Clematis fruticosa)苗木根系形态及养分承载对氮沉降的应激响应,以1年生盆栽灌木铁线莲为对象,分别采用单接种和混合接种,即:单接种根内根孢囊霉(Rhizophagus intraradices,以下简称+R),单接种摩西斗管囊霉(Funneliformis mosseae,以下简称+F);混合接菌(上述2菌种菌剂按体积1∶1混合,以下简称+RF)的菌根苗。以非菌根苗(未接菌,以下简称-M)为对照。氮沉降处理设置4个梯度(不施氮(0N,0 g·m-2·a-1)、低氮(LN,3 g·m-2·a-1)、中氮(MN,6 g·m-2·a-1)、高氮(HN,9 g·m-2·a-1)),1年后测定各处理细根形态(直径≤0.5 mm的总根长、总表面积、总体积、根尖数量)、菌根侵染率、土壤孢子密度及根、茎、叶各器官的养分(碳、氮、磷)含量等指标。①在+R和+RF处理下,LN处理的苗木菌根侵染率和孢子密度达到最大,且LN处理的苗木菌根侵染率显著高于HN处理;而+F处理的苗木菌根侵染率随氮沉降递增无显著差异。②0N处理下,+F和+R处理的灌木铁线莲苗木细根(直径≤0.5 mm)的总根长、总表面积、总体积和根尖数量均显著高于-M处理。然而,+F和+R处理的灌木铁线莲苗木上述根系形态指标随着氮沉降量的增加均呈下降的趋势。③+F和+R处理下,苗木养分承载量随氮沉降量增加呈增加的趋势。氮沉降处理下,接菌处理的苗木碳、氮、磷养分含量显著高于-M处理,其中+F处理下苗木碳氮磷养分含量最高。④直径≤0.5 mm细根形态指标与养分含量指标均呈正相关关系。综上,接菌处理可改变灌木铁线莲苗木细根形态对氮沉降的响应规律,接种摩西斗管囊霉有效增强苗木对氮沉降的适应能力,提高了高氮沉降处理下苗木的养分承载量。
中图分类号:
李赵毅, 郝龙飞, 刘婷岩, 何炎红, 张友, 白淑兰, 杨昕瑜. 接种丛枝菌根真菌对模拟大气氮沉降下灌木铁线莲根系形态及养分承载的影响[J]. 植物研究, 2022, 42(5): 886-895.
Zhaoyi LI, Longfei HAO, Tingyan LIU, Yanhong HE, You ZHANG, Shulan BAI, Xinyu YANG. AM Fungi Inoculation on Root Morphology and Nutrient Loading of Clematis fruticosa Seedlings under Simulated Atmospheric Nitrogen Deposition[J]. Bulletin of Botanical Research, 2022, 42(5): 886-895.
表2
模拟氮沉降及接菌处理对灌木铁线莲苗木菌根侵染率和孢子密度的影响
处理 Treatments | 菌根侵染率 Mycorrhizal colonization rate /% | 孢子密度 Spore density /(个/g) | |
|---|---|---|---|
接种根内根孢囊霉 (+R) | 不施氮处理(0N) | 86.22±1.11Ba | 777.67±38.41BCb |
| 低氮处理(LN) | 94.66±1.33Aab | 1 971.33±152.73Ab | |
| 中氮处理(MN) | 86.67±2.70Bb | 836.33±88.91Bb | |
| 高氮处理(HN) | 86.22±1.46Bab | 477.00±72.83Cb | |
接种摩西斗管囊霉 (+F) | 不施氮处理(0N) | 90.22±2.84Aa | 1 163.00±95.04BCa |
| 低氮处理(LN) | 92.22±0.97Ab | 2 551.00±157.10Aa | |
| 中氮处理(MN) | 94.89±1.35Aa | 1 502.67±119.33Ba | |
| 高氮处理(HN) | 91.11±1.46Aa | 905.67±63.17Ca | |
混合接菌 (+RF) | 不施氮处理(0N) | 92.22±1.46Ba | 539.00±108.17Bb |
| 低氮处理(LN) | 98.22±1.11Aa | 1 240.00±104.29Ac | |
| 中氮处理(MN) | 90.44±0.59Bab | 563.00±138.03Bb | |
| 高氮处理(HN) | 85.11±1.55Cb | 376.00±9.17Bb | |
表3
氮沉降和接菌处理对灌木铁线莲苗木细根(直径≤0.5 mm)形态的影响
处理 Treatments | 总根长 Root length /cm | 总表面积 Root surface area /cm² | 总体积 Root volume /cm3 | 根尖数量 /个 Tips | |
|---|---|---|---|---|---|
未接菌 (-M) | 不施氮处理(0N) | 331.84±27.80Ac | 36.12±3.10Ac | 0.34±0.03Ab | 372.33±39.68Bc |
| 低氮处理(LN) | 372.25±31.66Ac | 40.90±1.96Ac | 0.38±0.04Ab | 458.33±36.45ABc | |
| 中氮处理(MN) | 360.40±19.77Ac | 37.98±2.37Ac | 0.35±0.02Ac | 546.67±37.90Ab | |
| 高氮处理(HN) | 297.60±13.30Ab | 34.16±1.14Ab | 0.27±0.04Ac | 370.00±28.57Bc | |
接种根内根孢囊霉 (+R) | 不施氮处理(0N) | 797.33±31.88Aa | 81.39±4.61Aa | 0.72±0.05Aa | 1 138.00±64.45Aa |
| 低氮处理(LN) | 589.98±74.24Bab | 61.47±4.38Bb | 0.56±0.07ABab | 866.33±96.47Ab | |
| 中氮处理(MN) | 546.98±16.58Bb | 58.73±1.79Bb | 0.53±0.06ABb | 917.33±93.87Ab | |
| 高氮处理(HN) | 568.99±21.45Ba | 52.41±5.18Ba | 0.49±0.05Ba | 932.33±80.77Ab | |
接种摩西斗管囊霉 (+F) | 不施氮处理(0N) | 546.03±44.37Ab | 57.36±4.17Ab | 0.59±0.05Aa | 877.33±80.86Ab |
| 低氮处理(LN) | 434.21±48.99ABbc | 47.04±1.84Bc | 0.41±0.02Bb | 816.67±15.90ABb | |
| 中氮处理(MN) | 332.57±35.41Bc | 36.67±1.30Cc | 0.31±0.03Bc | 628.67±88.98BCb | |
| 高氮处理(HN) | 325.26±44.57Bb | 34.48±2.45Cb | 0.31±0.02Bbc | 475.00±24.83Cc | |
混合接菌 (+RF) | 不施氮处理(0N) | 409.35±51.72Cc | 42.32±4.79Bc | 0.39±0.04Cb | 726.67±107.60Cb |
| 低氮处理(LN) | 753.10±71.89ABa | 77.20±6.56Aa | 0.70±0.10ABa | 1 606.67±112.14Aa | |
| 中氮处理(MN) | 841.89±88.40Aa | 85.61±3.41Aa | 0.77±0.08Aa | 1 873.00±171.80Aa | |
| 高氮处理(HN) | 593.68±52.15BCa | 55.46±3.61Ba | 0.47±0.08BCab | 1 183.67±96.89Ba | |
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