柠檬醛猴樟茎段组织培养技术研究

doi:10.7525/j.issn.1673-5102.2020.02.005

植物研究 ›› 2020, Vol. 40 ›› Issue (2): 196-201.doi: 10.7525/j.issn.1673-5102.2020.02.005

柠檬醛猴樟茎段组织培养技术研究

肖祖飞^1,2, 王玲玲¹, 曹璐瑶¹, 廖文轩¹, 金志农¹, 李凤¹, 吕雄伟¹, 张北红¹, 赵姣¹

1. 南昌工程学院江西省樟树繁育与开发利用工程研究中心, 南昌 330099;
2. 江西省科学院生物资源研究所, 南昌 330096

收稿日期:2019-07-31 出版日期:2020-03-05 发布日期:2020-03-06
通讯作者: 金志农,E-mail:camphor.tree@qq.com E-mail:camphor.tree@qq.com
作者简介:肖祖飞(1983-),男,讲师,博士,主要从事樟树栽培与繁育研究。
基金资助:
江西省教育厅科技项目（GJJ151114）；国家自然科学基金（31600497）；江西省科技厅重点研发计划重点项目（20171ACH80016，20181ACF60002）；江西省科技厅重点研发计划一般项目（20192BBFL60012）

Tissue Culture Technology of Stem Segment of Cinnamomum bodinieri var. citralifera

XIAO Zu-Fei^1,2, WANG Ling-Ling¹, CAO Lu-Yao¹, LIAO Wen-Xuan¹, JIN Zhi-Nong¹, LI Feng¹, Lü Xiong-Wei¹, ZHANG Bei-Hong¹, ZHAO Jiao¹

1. Nanchang institute of technology, Jiangxi Porvincial Engineering Reseach Center of Seed-breeding and Utilization of Camphor Trees, Nanchang 330099;
2. Institute of biology and resources, Jiangxi academy of sciences, Nanchang 330096

Received:2019-07-31 Online:2020-03-05 Published:2020-03-06
Supported by:
Department of education of Jiangxi province science and technology innovation project(GJJ151114);National natural science foundation project(31600497);Department of science and technology of Jiangxi province key research and development of key project(20171ACH80016,20181ACF60002);Department of science and technology of Jiangxi province key research and development of general project(20192BBFL60012)

摘要/Abstract

摘要： 柠檬醛猴樟是重要的园林绿化、用材、油用树种。以柠檬醛猴樟一年生枝条为材料，研究采集季节、消毒时间、激素对柠檬醛猴樟茎段组织培养的影响。结果表明：5月中旬，柠檬醛猴樟半木质化茎段组织培养用浓度为0.1% HgCl₂消毒5 min，茎段腋芽萌芽率最高，褐化率和污染率较低。猴樟茎段萌芽适宜培养基为MS+1.0 mg·L^-1 6-BA+0.05 mg·L^-1 IBA，萌芽率为60%。增殖适宜培养基为MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 IBA，增殖系数为4.33、株高为3.67 cm、地径为1.04 mm。生根培养11 d基部出现不定根，生根适宜培养基为MS+1.5 mg·L^-1 IBA，生根率75.00%、根长5.06 cm、根数3.50、根粗0.82 mm。根数大于3的组培苗，移栽成活率80%以上。

关键词: 柠檬醛猴樟, 茎段, 组织培养

Abstract: Cinnamomum bodinieri var. citralifera is an important tree species for landscaping, timber and oil. We studied the effects of collection season, disinfection time and hormones on tissue culture of annual branches of C.bodinieri var. citralifera. The axillary bud germination rate of stem segments from semi-lignified stem segments of C.bodinieri var. citralifera was highest with 0.1% HgCl₂ disinfected for 5 min. in the Mid-May, and the browning rate and pollution rate were low. The suitable medium for stem segment germination was MS+1.0 mg·L^-1 6-BA+0.05 mg·L^-1 IBA, with germination ratio 60%. The suitable medium for multiplication was MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 IBA, with multiplication coefficient of 4.33, plant height of 3.67 cm and ground diameter of 1.04 mm. Adventitious roots appeared at 11 d after culture of the base of tissue culture seedlings. The suitable medium for rooting was MS+1.5 mg·L^-1 IBA. The rooting rate was 75.00%, the length of roots was 5.06 cm, the number of roots was 3.50, and the diameter of roots was 0.82 mm. Tissue culture seedlings with roots more than 3 had a survival rate of more than 80%.

Key words: Cinnamomum bodinieri var. citralifera, stem segment, tissue culture

中图分类号:

S792.23

肖祖飞, 王玲玲, 曹璐瑶, 廖文轩, 金志农, 李凤, 吕雄伟, 张北红, 赵姣. 柠檬醛猴樟茎段组织培养技术研究[J]. 植物研究, 2020, 40(2): 196-201.

XIAO Zu-Fei, WANG Ling-Ling, CAO Lu-Yao, LIAO Wen-Xuan, JIN Zhi-Nong, LI Feng, Lü Xiong-Wei, ZHANG Bei-Hong, ZHAO Jiao. Tissue Culture Technology of Stem Segment of Cinnamomum bodinieri var. citralifera[J]. Bulletin of Botanical Research, 2020, 40(2): 196-201.

参考文献 16

[1]	杨成华,任远.优良园林树种猴樟及其培育[J].贵州林业科技,2001,29(1):28-31.Yang C H,REN Y.Elite tree species for landscape-Cinnamomum bodinieri and it's cultivation[J].Guizhou Forestry Science and Technology,2001,29(1):28-31.
[2]	中国科学院中国植物志编辑委员会.中国植物志(第31卷)[M].北京:科学出版社,1982:174-176.Editorial Committee of Flora of China.Chinese Academy of Sciences.Flora of China(vol.31)[M].Beijing:Science Press,1982:174-176.
[3]	田小琴,韦小丽.生态环境对贵州猴樟人工林生长的影响[J].贵州农业科学,2011,39(4):184-187.Tian X Q,Wei X L.Effect of different ecological environments on plantation growth of Cinnamomum bodinieri in Guizhou[J].Guizhou Agricultural Sciences,2011,39(4):184-187.
[4]	张怡,韦小丽,王娈,等.不同地理种源猴樟苗期生长变异性[J].西南农业学报,2014,27(5):2162-2167.Zhang Y,Wei X L,Wang L,et al.Growth variability of Cinnamomum bodinieri seedlings from different geographical provenances[J].Southwest China Journal of Agricultural Sciences,2014,27(5):2162-2167.
[5]	陈瀚林,韦小丽.不同类型猴樟混交林群落的特征分析[J].贵州农业科学,2011,39(12):196-201.Chen H L,Wei X L.Analysis on community characteristics in different mixed forests of Cinnamomum bodinieri[J].Guizhou Agricultural Sciences,2011,39(12):196-201.
[6]	宋芳琳,张苗苗,苏金乐,等.自然降温过程中猴樟和香樟幼苗的抗寒性比较[J].西部林业科学,2012,41(6):48-52.Song F L,Zhang M M,Su J L,et al.The comparison on cold resistance between Cinnamomum bodinieri and Cinnamomum camphora seedlings in natural decreasing process of air temperature[J].Journal of West China Forestry Science,2012,41(6):48-52.
[7]	仇明华,姚祖凤,陈立义.湘西猴樟油主要化学成份分离研究[J].吉首大学学报:自然科学,1990,11(6):81-84.Qiu M H,Yao Z F,Chen L Y.Separating 1.8-cineole from Cinnamomum bodinieri lev oil[J].Journal of Jishou University,1990,11(6):81-84.
[8]	姚瑶,李勇鹏,杜丽.香樟成年态茎段组织培养的研究[J].河南农业科学,2014,43(12):125-127,148.Yao Y,Li Y P,Du L.Research on mature stem culture of Cinnamomum camphora L.[J].Journal of Henan Agricultural Sciences,2014,43(12):125-127,148.
[9]	何月秋,王建军.香樟涌金的组织培养和植株再生[J].南方农业学报,2015,46(1):96-100.He Y Q,Wang J J.Tissue culture and plant regeneration of Cinnamomum camphora Yongjin[J].Journal of Southern Agriculture,2015,46(1):96-100.
[10]	黄建,闵炜.樟树幼叶愈伤组织诱导培养基的筛选[J].江西农业大学学报,1993,15(4):393-396.Huang J,Min W.Selection of medium for the callus induction from the tender leaves of Cinnamomum camphora[J].Acta Agriculturae Universitatis Jiangxiensis,1993,15(4):393-396.
[11]	周丽华,蔡燕灵,曾令海,等.樟树优良家系的组培育苗技术研究[J].热带作物学报,2013,34(1):67-73.Zhou L H,Cai Y L,Zeng L H,et al.Tissue culture and micropropagation of superior families of Cinnamomum camphora(L.) presl[J].Chinese Journal of Tropical Crops,2013,34(1):67-73.
[12]	蔡玲,覃子海,黄金使,等.樟脑型樟树芽器官离体培养技术[J].广西林业科学,2013,42(4):315-318.Cai L,Qin Z H,Huang J S,et al.Culture technology of buds in Cinnamomum camphora with camphor in vitro[J].Guangxi Forestry Science,2013,42(4):315-318.
[13]	连芳青,熊伟,张露.樟树茎段的离体培养和植株再生[J].江西林业科技,1992(3):20-21.Lian F Q,Xiong W,Zhang L.In vitro culture and plantlet regeneration of stem segments of Cinnamomum camphora[J].Jiangxi Forestry Science and Technology,1992(3):20-21.
[14]	彭东辉.脑樟组织培养技术研究[J].福建林学院学报,2005,25(4):313-317.Peng D H.Studies on tissue culture of Cinnamomum camphora[J].Journal of Fujian College of Forestry,2005,25(4):313-317.
[15]	吴幼媚,王以红,蔡铃,等.香樟优良无性系快繁技术的研究[J].广西农业生物科学,2006,25(1):60-64.Wu Y M,Wang Y H,Cai L,et al.Research of rapid propagation and tissue culture of elite clone Cinnamomum camphora[J].Journal of Guangxi Agricultural and Biological Science,2006,25(1):60-64.
[16]	洪立萍.芳樟的离体繁殖[J].林业科技,2007,32(4):1-2.Hong L P.In vitro propagation of Cinnamomum camphora[J].Forestry Science & Technology,2007,32(4):1-2.

柠檬醛猴樟茎段组织培养技术研究

Tissue Culture Technology of Stem Segment of Cinnamomum bodinieri var. citralifera

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