长期氮添加对东北地区兴安落叶松人工林土壤酶的影响

doi:10.7525/j.issn.1673-5102.2019.04.014

植物研究 ›› 2019, Vol. 39 ›› Issue (4): 598-603.doi: 10.7525/j.issn.1673-5102.2019.04.014

长期氮添加对东北地区兴安落叶松人工林土壤酶的影响

马鹏宇¹, 张红光², 昝鹏¹, 顾伟平¹, 温璐宁¹, 张子嘉³, 翁海龙⁴, 孙涛³, 毛子军¹

1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
2. 东北林业大学帽儿山实验林场, 尚志 150611;
3. 中国科学院森林生态与管理重点实验室(沈阳应用生态研究所), 沈阳 110016;
4. 黑龙江丰林国家级自然保护区管理局, 五营 153033

收稿日期:2018-11-26 出版日期:2019-07-05 发布日期:2019-07-03
通讯作者: 毛子军 E-mail:zijunm@nefu.edu.cn
作者简介:马鹏宇(1993-),女,硕士研究生,主要从事全球变化生态学研究。
基金资助:
国家自然科学基金（41312304，31500361和31700397）；中央高校基本科研业务费专项资金项目（2572017AA21）

Effects of Long-term Nitrogen Addition on Soil Enzymes in Larix gmelinii Plantation in Northeast China

MA Peng-Yu¹, ZHANG Hong-Guang², ZAN Peng¹, GU Wei-Ping¹, WEN Lu-Ning¹, ZHANG Zi-Jia³, WENG Hai-Long⁴, SUN Tao³, MAO Zi-Jun¹

1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
2. Maoershan Experimental Forest Station of Northeast Forestry University, Shangzhi 150611;
3. Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016;
4. Heilongjiang Fenglin National Nature Reserve Administration, Wuying 153033

Received:2018-11-26 Online:2019-07-05 Published:2019-07-03
Supported by:
National Science Foundation of China(41312304,31500361 and 31700397);Fundamental Research Funds for the Central Universities(2572017AA21)

摘要/Abstract

摘要： 全球氮沉降持续增加导致的氮沉降量上升，已成为森林中物质循环的重要生态因子。为探讨氮沉降增加对东北地区兴安落叶松人工林土壤酶的影响，对长期人工添加无机氮（NH₄NO₃）和未添加无机氮的兴安落叶松人工林土壤酶活性进行了比较分析。实验分为2个处理，分别为对照处理（CK：0 kg·N·hm^-2·a^-1）和无机氮处理（IN：10 kg·N·hm^-2·a^-1），每个处理含3个重复。结果表明，在连续8年添加无机氮的情况下，β-1，4-糖苷酶（BG）、酸性磷酸酶（AP）和过氧化物酶（PER）活性上升（5.56%、8.66%和0.61%）；纤维素二糖水解酶（CBH）、亮氨酸氨基肽酶（LAP）和多酚氧化酶（POX）活性下降（10.71%、3.85%和12.30%）。与之相对应的土壤碳、氮和磷元素含量也有变化（0.80%、12.66%和-2.25%）。因此，在氮沉降量逐年增加的情况下，研究土壤酶活性变化，能更好的解释森林生态系统物质循环特性，理解生物地球物质循环机理并预测未来土壤碳库变化。

关键词: 氮沉降, 土壤酶活性, 物质循环, 兴安落叶松, 人工林

Abstract: The increase of nitrogen deposition hadbecame a significant ecological factor effecting the material cycle in forest. We examined the effects of nitrogen deposition on soil enzyme activities in inorganic nitrogen addition and control plots in Larix gmelinii(Rupr.) Rupr. plantation, in northeast China. The experiment was divided into two treatment groups, one was control treatment(CK:0 kg·N·hm^-2·a^-1) and another was inorganic nitrogen treatment(IN:10 kg·N·hm^-2·a^-1). Each treatment included 3 replicate plots. Over 8 years of inorganic nitrogen addition, our results showed that N fertilization increased the activities of β-1, 4-glucosidase(BG), acid phosphatase(AP), and peroxidase(PER)(5.56%, 8.66% and 0.61%)while reduced the activities of cellobiohydrolase(CBH), leucine aminopeptidase(LAP) and phenol oxidases(POX)(10.71%, 3.85% and 12.30%). The long-term inorganic nitrogen addition treatment had a significant effect on soil enzyme activity and soil carbon, nitrogen and phosphorus cycles(0.80%, 12.66% and -2.25%). Therefore, it is important to include the soil enzyme activity when studying the response of biogeochemical cycle and soil carbon dynamics to climate changein forest ecosystems in future research, while nitrogen deposition increases year by year.

Key words: nitrogen deposition, soil enzyme activity, material cycle, Larix gmelinii(Rupr.) Rupr., plantation

中图分类号:

S791.222

马鹏宇, 张红光, 昝鹏, 顾伟平, 温璐宁, 张子嘉, 翁海龙, 孙涛, 毛子军. 长期氮添加对东北地区兴安落叶松人工林土壤酶的影响[J]. 植物研究, 2019, 39(4): 598-603.

MA Peng-Yu, ZHANG Hong-Guang, ZAN Peng, GU Wei-Ping, WEN Lu-Ning, ZHANG Zi-Jia, WENG Hai-Long, SUN Tao, MAO Zi-Jun. Effects of Long-term Nitrogen Addition on Soil Enzymes in Larix gmelinii Plantation in Northeast China[J]. Bulletin of Botanical Research, 2019, 39(4): 598-603.

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长期氮添加对东北地区兴安落叶松人工林土壤酶的影响

Effects of Long-term Nitrogen Addition on Soil Enzymes in Larix gmelinii Plantation in Northeast China

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