四川桤木7×7完全双列杂交种实、种苗性状的遗传效应分析

doi:10.7525/j.issn.1673-5102.2018.03.007

植物研究 ›› 2018, Vol. 38 ›› Issue (3): 357-366.doi: 10.7525/j.issn.1673-5102.2018.03.007

四川桤木7×7完全双列杂交种实、种苗性状的遗传效应分析

郭洪英^1,2, 杨汉波², 陈炙², 王泽亮², 黄振², 李佳蔓², 肖兴翠², 康向阳¹

1. 林木分子设计育种高精尖创新中心/林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/城乡生态环境北京实验室, 北京林业大学生物科学与技术学院, 北京 100083;
2. 四川省林业科学研究院, 成都 610081

收稿日期:2017-10-30 出版日期:2018-05-15 发布日期:2018-05-17
通讯作者: 康向阳,E-mail:kangxy@bjfu.edu.cn E-mail:kangxy@bjfu.edu.cn
作者简介:郭洪英(1973-),女,博士,研究员,主要从事林木遗传育种方面的研究工作。
基金资助:
四川省科技计划项目—突破性林木育种材料与方法创新（2016NYZ0035）；四川省科技计划项目：林—板—家具一体化现代产业链关键技术集成研究与产业化示范（2014NZ0033）、桤木杂交子代苗木培育及苗期评价研究（ZL2017-02）

Genetic Effects of Fruit, Seed and Seedling Traits of Alnus cremastogyne Burk in a 7×7 Complete Diallel Cross Design

GUO Hong-Ying^1,2, YANG Han-Bo², CHEN Zhi², WANG Ze-Liang², HUANG Zhen², LI Jia-Man², XIAO Xing-Cui², KANG Xiang-Yang¹

1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design/National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants/Beijing Laboratory of Urban and Rural Ecological Environment, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083;
2. Sichuan Academy of Forestry, Chengdu 610081

Received:2017-10-30 Online:2018-05-15 Published:2018-05-17
Supported by:
Scientific and technological projects of Sichuan-Radical innovation of forest tree breeding materials and methods(2016NYZ0035); Scientific and technological projects of Sichuan:Critical technology integrated research and industrialization demonstration of wood-board-furniture integration of modern industrial chain(2014NZ0033), Seedling cultivation and evaluation of alder hybrid progeny(ZL2017-02)

摘要/Abstract

摘要： 通过对四川桤木杂交子代果实、种子及种苗性状的分析，估算四川桤木亲本的遗传参数，为四川桤木杂交育种亲本选配提供理论依据。以7个优树作为杂交亲本，按7×7完全双列交配设计进行控制授粉，测定49个杂交组合的果实、种子及苗木性状。结果表明，49个杂交组合间的果实鲜重、种子千粒重、果长、种长等表型性状及苗高、地径均存在着极显著的差异（P<0.001）；果实鲜重、种子千粒重、果长、种长等表型性状的一般配合力（GCA）、特殊配合力（SCA）和反交效应差异（REC）也存在着极显著的差异（P<0.001）。苗高和地径的GCA、SCA和REC也存在着极显著差异（P<0.001）。苗高和地径的广义遗传力分别为37.06%和38.04%，狭义遗传力分别为17.47%和16.03%，表明在苗期阶段，苗木生长受环境效应的影响较大。种实性状的加性方差是非加性方差的1.34~11.79倍，主要受加性基因效应控制，非加性效应次之。而苗高和地径的非加性方差分别是加性方差的1.12和1.37倍，主要受非加性基因效应影响，加性效应次之。综合分析杂交子代GCA、SCA和REC效应值，确定JG6和JT4为优良亲本，从49个杂交组合中初选出4个组合（BZ6×JG6、BZ6×SW2、JT4×TT13和BZ6×QJ1）为优良杂交组合，其杂交子代苗高和地径分别实现20.47%~76.22%和5.07%~43.18%的遗传增益。

关键词: 四川桤木, 完全双列杂交, 一般配合力, 特殊配合力, 遗传增益

Abstract: The genetic parameters of parents were estimated by analyzing its hybrids descendant for fruit, seed and seedling traits to select optimum parents for crossbreeding of Alnus cremastogyne Burk. Seven parents of A. cremastogyne were crossed by a 7×7 diallel cross design and their hybrid fruits, seeds and seedlings were tested for fresh weight, thousand seed weight, phenotypic characters like fruit and seed length, seedling height, and caliper. The results showed that there were very significant differences in the characteristics of fruit fresh weight, thousand kernel weight, phenotype character of fruit and seed, seedling height, and caliper between 49 cross groups(P<0.001). In the general combining ability(GCA), specific combining ability(SCA) and reciprocal effects(REC) of fruit fresh weight, thousand kernel weight, and phenotype character of fruit and seed(P<0.001). There also were very significant differences of GCA, SCA, and REC for seedling height and caliper(P<0.001). The broad-sense heritability of seedling height and caliper were 37.06% and 38.04%, and the narrow-sense heritability of seedling height and caliper were 17.47% and 16.03%, respectively. That means higher environment effects existed in the stage of seedling growth. The additive variance of fruit and seed characters were from 1.34 to 11.79 times to non-additive variance, which mainly controlled by additive gene effect, followed by non-additive effect. Otherwise, the non-additive variance of seedling height and caliper were 1.12 and 1.37 times to additive variance, which mainly controlled by non-additive gene effect, followed by additive effect. JG6 and JT4 are superior parents according to the effects of GCA, SCA, and REC. Also, four groups(BZ6×JG6, BZ6×SW2, JT4×TT13 and BZ6×QJ1) are superior cross groups, which hybrids descendants get 20.47%-76.22% genetic gain on seedling height and 5.07%-43.18% genetic gain on the caliper, respectively.

Key words: Alnus cremastogyne Burk, complete diallel cross, GCA, SCA, genetic gain

中图分类号:

S792.14

郭洪英, 杨汉波, 陈炙, 王泽亮, 黄振, 李佳蔓, 肖兴翠, 康向阳. 四川桤木7×7完全双列杂交种实、种苗性状的遗传效应分析[J]. 植物研究, 2018, 38(3): 357-366.

GUO Hong-Ying, YANG Han-Bo, CHEN Zhi, WANG Ze-Liang, HUANG Zhen, LI Jia-Man, XIAO Xing-Cui, KANG Xiang-Yang. Genetic Effects of Fruit, Seed and Seedling Traits of Alnus cremastogyne Burk in a 7×7 Complete Diallel Cross Design[J]. Bulletin of Botanical Research, 2018, 38(3): 357-366.

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四川桤木7×7完全双列杂交种实、种苗性状的遗传效应分析

Genetic Effects of Fruit, Seed and Seedling Traits of Alnus cremastogyne Burk in a 7×7 Complete Diallel Cross Design

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