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植物研究 ›› 2013, Vol. 33 ›› Issue (3): 287-293.doi: 10.7525/j.issn.1673-5102.2013.03.007

• 论文 • 上一篇    下一篇

太空诱变对迷迭香(Rosmarinus officinalis L.)形态参数、光合能力和次生代谢产物含量和成分的影响

王洪政1,2;郭晓瑞1,2;唐中华1,2;于景华1,2;祖元刚1,2;张学科1,2*   

  1. 1.东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040;2.生物资源生态利用国家地方联合工程实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-20 发布日期:2013-05-20
  • 通讯作者: 张学科
  • 基金资助:
     

Effect of Space Mutantion on Morphological Parameters,Photosynthetic Capacities as well as Contents and Compositions of Secondary Metabolites of Rosmarinus officinalis L.

WANG Hong-Zheng;GUO Xiao-Rui;TANG Zhong-Hua;YU Jing-Hua;ZU Yuan-Gang;ZHANG Xue-Ke;*   

  1. 1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;2.State Engineering Laboratory of BioResources EcoUtilization,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: ZHANG Xue-Ke
  • Supported by:
     

摘要: 以未经太空诱变的迷迭香(Rosmarinus officinalis L.)为对照,研究了经返回式卫星搭载进行太空诱变后迷迭香在形态参数、光合作用以及次生产物代谢方面的变化。结果显示:太空诱变后,迷迭香优良株系的株高、冠幅、分枝数、分枝长、地上部生物量等形态参数有了明显提高,特别是反映迷迭香可采收量的地上部生物量,比诱变前提高了1.24倍。进一步研究发现,光合能力的提升是迷迭香诱变株系形态参数发生变化的一个重要原因,因为迷迭香诱变株系的叶绿素含量、最大净光合速率、表观量子效率比对照明显提高,而光补偿点则明显下降。作为迷迭香中最主要的生物活性物质,鼠尾草酸、迷迭香酸和精油的含量在太空诱变株系中明显高于对照,并且精油中主要组分的比例也发生了显著变化。上述结果表明,太空诱变中迷迭香可获得生物产量和经济产量的有益突变,从而证明航天育种技术在迷迭香优良品种选育中是可行的。

关键词: 迷迭香, 形态参数, 光合作用, 次生代谢

Abstract: Taking the rosemary (Rosmarinus officinalis L.) without mutation as control, the changes of morphological parameters, photosynthesis and secondary metabolites in the line mutated by the retrievable satellite were investigated. The results showed that the morphological parameters of rosemary, including plant height, canopy size, number of branches, branch length and aboveground biomass, were significantly increased by the space mutation. Especially, the aboveground biomass of mutant line, which restricted the recoverable amount of rosemary, was 1.24 times than the control. Further study demonstrated that the improvement of photosynthesis capacity was an important reason of the change of morphological parameters in mutant rosemary, because significant increasing of chlorophyll content, maximum net photosynthesis rate, apparent quantum yield, and declining of light compensation point were found in mutant rosemary. The contents of carnosic acid, rosmarinic acid and essential oil, three mayor biological active substances, were significantly higher in mutant rosemary than in control. In addition, differences in component proportion were detected between the essential oils from mutant line and control. All the above results indicated that beneficial mutation of both biological and economic yields could be obtained in space mutagenesis of rosemary. It means that space mutagenesis breeding is feasible for rosemary.

Key words: Rosmarinus officinalis L., morphological parameters, photosynthesis, secondary metabolism

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