新型真菌源激活蛋白诱导水稻抗病性及其生理机制

doi:10.7525/j.issn.1673-5102.2013.02.017

植物研究 ›› 2013, Vol. 33 ›› Issue (2): 220-224.doi: 10.7525/j.issn.1673-5102.2013.02.017

新型真菌源激活蛋白诱导水稻抗病性及其生理机制

袁肖寒^1,2；顾成波^1*；邱德文³；付丽楠¹；李旺¹；王秋雪¹；郭东杰¹；蔡曼¹

1.东北林业大学，哈尔滨　150040；2.东北农业大学生命科学与生物技术研究中心，哈尔滨　150030；3.中国农业科学院植物保护研究所,北京　100081

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-20 发布日期:2013-03-20
通讯作者: 顾成波
基金资助:

Rice Disease Resistance Induced by New Fungal Activator Protein and Its Physiological Mechanism

YUAN Xiao-Han;GU Cheng-Bo*;QIU De-Wen;FU Li-Nan;LI Wang;WANG Qiu-Xue;GUO Dong-Jie;CAI Man

1.Northeast Forestry University,Harbin　150040；2.Life Science and Biotechnique Research Center,Northeast Agricultural University,Harbin　150030；3.Key Laboratory for Biological Control of Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing　100081

Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
Contact: GU Cheng-Bo
Supported by:

摘要/Abstract

摘要： 为明确新型真菌源激活蛋白对水稻抗病性的诱导作用及其生理机制，研究了激活蛋白对水稻稻瘟病和白叶枯病的诱导抗病性，监测了激活蛋白处理后水稻过氧化物酶（POD）、多酚氧化酶（PPO）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性及过氧化氢（H₂O₂）含量变化。结果表明，1～6 μg·mL^-1激活蛋白对稻瘟病和白叶枯病的诱抗效果分别为45.2%～71.4%和47.6%～66.3%，以6 μg·mL^-1激活蛋白的诱抗效果最好。与对照相比，2 μg·mL^-1激活蛋白处理水稻后3～15 d内不同程度诱导了防御酶POD、PPO和SOD活性，抑制CAT活性，提高H₂O₂含量。新型真菌源激活蛋白能够诱导水稻产生对稻瘟病和白叶枯病的抗病性，其诱导抗性机制与水稻体内的活性氧代谢密切相关。

关键词: 激活蛋白, 水稻病害, 诱导抗性, 活性氧代谢

Abstract: In order to clarify new fungal activator protein induced resistance in rice and its physiological mechanism, changes of peroxidase (POD), polyphenol oxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activities and hydrogen peroxide (H₂O₂) content in rice seedlings were determined at various time points after activator protein treatment. The results showed that activator protein could induce resistance in rice against blast fungus and bacterial blight. The resistance induced by activate protein at the concentration of 1-6 μg·mL^-1 against blast fungus and bacterial blight was 45.2%-71.4% and 47.6%-66.3%, respectively, and the best results were achieved at the concentration of 6 μg·mL^-1 activate protein. Compared with control group, the activities of POD, PPO and SOD and hydrogen peroxide content in rice seedlings were increased with different levels, but CAT activities inhibited 3-15 d after the rice seedlings treated with the concentration of 2 μg·mL^-1 activator protein. These findings showed that activator protein-induced resistance in rice was closely related to active oxygen metabolism.

Key words: activator protein, rice disease, induced resistance, active oxygen metabolism

中图分类号:

S511

袁肖寒;顾成波*;邱德文;付丽楠;李旺;王秋雪;郭东杰;蔡曼. 新型真菌源激活蛋白诱导水稻抗病性及其生理机制[J]. 植物研究, 2013, 33(2): 220-224.

YUAN Xiao-Han;GU Cheng-Bo*;QIU De-Wen;FU Li-Nan;LI Wang;WANG Qiu-Xue;GUO Dong-Jie;CAI Man. Rice Disease Resistance Induced by New Fungal Activator Protein and Its Physiological Mechanism[J]. Bulletin of Botanical Research, 2013, 33(2): 220-224.

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新型真菌源激活蛋白诱导水稻抗病性及其生理机制

Rice Disease Resistance Induced by New Fungal Activator Protein and Its Physiological Mechanism

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