基于ITS区和AFLP的吴茱萸分子鉴定方法

doi:10.7525/j.issn.1673-5102.2010.03.004

植物研究 ›› 2010, Vol. 30 ›› Issue (3): 273-277.doi: 10.7525/j.issn.1673-5102.2010.03.004

基于ITS区和AFLP的吴茱萸分子鉴定方法

黄海¹;刘杨¹;冉贵萍¹;何顺志²;黄韵祝¹;徐文芬²;王良宏¹

1.贵阳医学院临床生化教研室，贵阳　550004；2.贵阳中医学院药学系，贵阳　550002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
基金资助:

Molecular Authentication Methods for Evodia rutaecarpa(Juss.) Benth Based on ITS Region and AFLP

HUANG Hai;LIU Yang;RAN Gui-Ping;HE Shun-Zhi;HUANG Yun-Zhu;XU Wen-Fen;WANG Liang-Hong

1.Department of Clinical Biochemistry,Guiyang Medical College,Guiyang　550004；2.Department of Traditional Chinese Medicine,Guiyang College of Traditional Chinese Medicine,Guiyang　550002

Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
Supported by:

摘要/Abstract

摘要： 吴茱萸作为一种重要的中药具有不同的变种，疏毛吴茱萸和石虎。AFLP技术和ITS区测序技术被用于鉴定14株吴茱萸、疏毛吴茱萸和石虎样品。ITS测序发现吴茱萸及其变种具有高度相似性，一致性达97%。在吴茱萸与其变种之间发现有一个SNP位点差异。扩增性片段长度多态性在它们之间具有差异条带。根据上述结果，分子鉴定方法能够用于鉴别吴茱萸及其变种。

关键词: 吴茱萸, 基因间隔序列, 扩增片段长度多态性, 分子鉴定

Abstract: Evodia rutaecarpa(Juss.) Benth is an important medicinal plant species that has diffierent varieties, namely E.rutaecarpa(Juss.) Benth. var. officinalis(Dode) Huang, and E.rutaecarpa(Juss.) Benth. var. bodinieri(Dode) Huang. AFLP technique and ITS region sequencing were used to distinguish 14 samples belonging to the three varieties of E.rutaecarpa(Juss.) Benth. Sequence analysis of the ITS regions amplified from the three varieties of E.rutaecarpa(Juss.) Benth. indicated that ITS regions were highly similar and the identities was more than 97%. A SNP nucleotide was discovered between E.rutaecarpa(Juss.) Benth. and its two varieties. Amplified fragment length polymorphism was compared among E.rutaecarpa(Juss.) Benth, E.rutaecarpa(Juss.) Benth. var. officinalis(Dode) Huang and E.rutaecarpa(Juss.) Benth. var. bodinieri(Dode) Huang and different specific bands appeared among three varieties of E.rutaecarpa(Juss.) Benth. Based on these specific markers, molecular authentication methods can be developed to identify and distinguish E.rutaecarpa(Juss.) Benth and its two varieties.

Key words: Evodia rutaecarpa(Juss.) Benth, internal transcribed spacer, amplified fragment length polymorphism, molecular authentication

中图分类号:

S567.21+9

黄海;刘杨;冉贵萍;何顺志;黄韵祝;徐文芬;王良宏. 基于ITS区和AFLP的吴茱萸分子鉴定方法[J]. 植物研究, 2010, 30(3): 273-277.

HUANG Hai;LIU Yang;RAN Gui-Ping;HE Shun-Zhi;HUANG Yun-Zhu;XU Wen-Fen;WANG Liang-Hong. Molecular Authentication Methods for Evodia rutaecarpa(Juss.) Benth Based on ITS Region and AFLP[J]. Bulletin of Botanical Research, 2010, 30(3): 273-277.

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基于ITS区和AFLP的吴茱萸分子鉴定方法

Molecular Authentication Methods for Evodia rutaecarpa(Juss.) Benth Based on ITS Region and AFLP

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