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植物研究 ›› 2008, Vol. 28 ›› Issue (2): 236-239.doi: 10.7525/j.issn.1673-5102.2008.02.022

• 论文 • 上一篇    下一篇

培养条件对黄檗快速繁殖影响的研究

张玉红;曲伟娣   

  1. (东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-20 发布日期:2008-03-20
  • 通讯作者: 张玉红
  • 基金资助:
     

Different Culture Conditions on Rapid Propagation of Phellodendron amurense

ZHANG Yu-Hong;QU Wei-Di   

  1. (Key Laboratory of Forestry Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20
  • Contact: ZHANG Yu-Hong
  • Supported by:
     

摘要: 以黄檗带顶芽和腋芽的茎段为材料,以MS和1/2MS为基本培养基,进行黄檗组培苗的快速繁殖,以研究不同培养条件对黄檗快速繁殖的影响。结果表明:以MS+6-BA 0.8 mg·L-1为培养基,浓度10%次氯酸钠溶液消毒黄檗茎段8 min,其组培苗生长最旺盛,成活率可达92.8%。以MS为基本培养基,蔗糖浓度20 mg·L-1,pH值5.8对黄檗组培苗的壮苗效果最好。

关键词: 黄檗, 快速繁殖, 培养条件

Abstract: Phellodendron amurense Rupr. stem segment with axillary bud and top bud were cultured with MS and 1/2MS medium in this experiment,in order to study the effects on rapid propagation of P. amurense in different culture conditions.The results showed that MS+6-BA0.8 mg·L-1 culture medium and taking antisepsis for stem segment with axillary’s bud with 10% javel water eight minutes were the best.The survival rate of transplanting reached to 92.8%.MS was the optimum medium type. Sucrose concentrations 20 g·L-1 and pH5.8 were the optimal culture condition for enhance culture of P. amurense seedling.

Key words: Phellodendron amurense Rupr., rapid propagation, culture conditions

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