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植物研究 ›› 2003, Vol. 23 ›› Issue (3): 280-284.

• 论文 • 上一篇    下一篇

球花石楠离体培养及快速繁殖研究

林萍, 普晓兰   

  1. 西南林学院, 昆明 650224
  • 收稿日期:2002-03-06 出版日期:2003-09-15 发布日期:2016-06-14
  • 作者简介:林萍(1958-),女,副教授,主要从事园林植物研究工作。

RAPID PROPAGATION OF PHOTINIA GLOMERATA IN VITRO

LIN Ping, PU Xiao-Lan   

  1. Southwest Forestry College, Kunming 650224
  • Received:2002-03-06 Online:2003-09-15 Published:2016-06-14

摘要: 取球花石楠的不同器官、不同部位的材料进行离体培养及快速繁殖研究。试验结果表明,根部萌蘖条和盆栽大苗的嫩茎为首选外植体,在MS+6-BA 2 mg·L-1+NAA 0.5 mg·L-1的培养基上有较高的腋芽萌发率,增殖阶段以MS+6-BA 4 mg·L-1+NAA 0.1 mg·L-1培养基上的小苗最多,生长健壮,生根可在1/2 MS+IBA 0.25 mg·L-1的培养基上进行。分析了生根较低的原因并提出改进措施。

关键词: 球花石楠, 离体培养, 快速繁殖, 外植体

Abstract: Rapid propagation of different parts from different organs of Photinia glomerata in vitro was studied. The results showed that the high germination rate of axially bud from the explants of stump shoot and potted seedling appears on the medium of MS+6-BA2 mg·L-1+NAA 0.5 mg·L-1. The most numerous and sturdy plantlet appears on the medium of MS+6-BA4mg·L-1+NAA 0.1 mg·L-1 during breeding. The roots grow well on the medium of 1/2 MS+IBA 0.25 mg·L-1. The reasons of lower strike root rate were discussed and the improved measures were put forward.

Key words: Photinia glomerata, culture in vitro, rapid propagation, explant