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植物研究 ›› 2003, Vol. 23 ›› Issue (1): 72-76.

• 论文 • 上一篇    下一篇

黑曲霉及其多糖组分诱导红豆杉叶子产生抗病反应之比较

李默怡, 余龙江, 陈超, 兰文智   

  1. 华中科技大学生命科学技术学院, 武汉 430074
  • 收稿日期:2002-10-16 出版日期:2003-03-15 发布日期:2016-06-14
  • 作者简介:李默怡(1978-),女,硕士,主要从事植物次生代谢途径分子生物学研究。

DIFFERENT EFFECTS OF ASPERGILLUS NIGER AND ITS POLYSCCHARIDES ON ELICITING DISEASE RESPONSES OF TAXUS CHINENESIS LEAVES

LI Mo-Yi, YU Long-Jiang, CHENG Chao, LAN Wen-Zhi   

  1. School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074
  • Received:2002-10-16 Online:2003-03-15 Published:2016-06-14

摘要: 用一种从中国红豆杉树皮内分离得到的黑曲霉及其酸解物多糖组分处理离体红豆杉叶子,发现经过两种不同处理的叶子干重和叶绿素含量均比对照下降,而丙二醛含量上升,但黑曲霉处理的呈现更为明显的趋势。说明多糖和黑曲霉孢子对叶片均有损伤,但多糖造成的损伤较小。多糖组分处理提高酚类物质和可溶性蛋白的含量并激活过氧化物酶和苯丙氨酸解氨酶的活性,而黑曲霉却导致了相反的现象,因此认为多糖组分具有诱导红豆杉叶子产生抗病反应的能力,而黑曲霉却具有致病性。

关键词: 红豆杉, 黑曲霉, 抗病反应

Abstract: A fungus Aspergillus niger strain isolated from the inner bark of Taxus chinensis and its hydrolyzed product polysaccharides were used to treat Taxus chinensis leaves. Both treatments decreased the dry weight and chlorophyll content of the leaves meanwhile increased malondialdehyde (MDA) content compared with the control, and fungi led to more distinguishable outcome than polysaccharides did, indicating that both fungus and fungal polysaccharides destroyed leaves while the latter had less damaging effects. Furthermore, Polysaccharides could notably increase content of protein and phenolics, evoke activity of peroxidase and phenylalanine ammonia-lyase, whereas the fungus had the contrary effects. Thus, it demonstrated that polysaccharides have inducing effects on defense reaction of Taxus chinensis while the fungus strain itself does not.

Key words: Taxus chinensis, Aspergillus niger, disease response