吸附剂及培养基组成对香荚兰细胞悬浮培养产生香兰素的影响

植物研究 ›› 2002, Vol. 22 ›› Issue (1): 65-67.

吸附剂及培养基组成对香荚兰细胞悬浮培养产生香兰素的影响

曹孟德¹, 李家儒², 秦东春¹, 王君健³

1. 郑州大学医学院, 郑州 450052;
2. 武汉大学生命科学院, 武汉 430072;
3. 华中理工大学生物工程系, 武汉 430074

收稿日期:2001-10-29 出版日期:2002-03-15 发布日期:2016-06-14
作者简介:曹孟德(1964-),男,博士,副教授,主要从事植物生物化学研究。

EFFECTS OF ABSORBENTS AND COMPOENTS OF MEDIUM ON VANILLIN BIOSYSTHESIS IN CELL CUTURES OF VANILLA PLANIFOLIA

CAO Meng-de¹, LI Jia-ru², QIN Dong-chun¹, WANG Jun-jian³

1. The Medical college of Zhengzhou University, Zhengzhou 450052;
2. Department of Biology, Wuhan University, Wuhan 430072;
3. Bioengineering Department, Huazhong University of Science and Technology, Wuhan 430074

Received:2001-10-29 Online:2002-03-15 Published:2016-06-14

摘要/Abstract

摘要： 本文报道了培养基的组成及添加吸附剂对香荚兰细胞悬浮培养产生香兰素的影响。结果表明,添加吸附剂活性炭及XAD-2后,香荚兰细胞产生的香兰素含量明显增加,而且活性炭的效果优于XAD-2;香荚兰细胞在全组成的MS培养基中香兰素含量均低于由矿物质盐组成培养基中的含量。可以考虑采用二步培养法来培养香荚兰细胞及产生香兰素。

关键词: 香荚兰, 香兰素, 吸附剂, 细胞培养

Abstract: This paper reported the effects of absorbents and composition of medium on vanillin biosynthesis in cell cultures of Vanilla planifonia. It was shown that the absorbents XAD-2 and activated charcoal had a marked increasing effect on the accumulation of vanillin, and the activated charcoal had more marked increasing effect than XAD-2;and that the contents of vanillin of Vanilla planifonia cells cultured on the complete MS medium were lower than that on the medium composed of mineral salts. It was suggested that two-step culture should be taken during the production of vanillin of Vanilla planifonia cells in batch suspension culture.

Key words: Vanilla planifonia, vanillin, absorbent, cell culture

曹孟德, 李家儒, 秦东春, 王君健. 吸附剂及培养基组成对香荚兰细胞悬浮培养产生香兰素的影响[J]. 植物研究, 2002, 22(1): 65-67.

CAO Meng-de, LI Jia-ru, QIN Dong-chun, WANG Jun-jian. EFFECTS OF ABSORBENTS AND COMPOENTS OF MEDIUM ON VANILLIN BIOSYSTHESIS IN CELL CUTURES OF VANILLA PLANIFOLIA[J]. Bulletin of Botanical Research, 2002, 22(1): 65-67.

[1]	曹孟德, 李家儒, 王君健. 诱导物及前体物对香荚兰细胞悬浮培养产生香兰素的影响[J]. 植物研究, 2000, 20(3): 266-269.
[2]	李家儒, 曹孟德, 刘曼西, 陈辉蓉, 吴振斌, 王君健. 前体物对红豆杉培养细胞中紫杉醇生物合成的影响[J]. 植物研究, 1999, 19(3): 356-360.
[3]	李家儒, 刘曼西, 曹孟德, 王君健. 桔青霉诱导子对红豆杉培养细胞中紫杉醇生物合成的影响[J]. 植物研究, 1998, 18(1): 78-82.