小麦与禾草对称融合中杂种染色体组成与亲缘关系的初步探究

植物研究 ›› 2001, Vol. 21 ›› Issue (1): 74-78.

小麦与禾草对称融合中杂种染色体组成与亲缘关系的初步探究

邢梅青¹, 夏光敏², 陈惠民²

1. 山东省实验中学, 济南 250001;
2. 山东大学, 济南 250100

收稿日期:2000-09-20 出版日期:2001-03-15 发布日期:2016-06-14
作者简介:邢梅青(1968-), 女, 中教一级(硕士), 从事植物发育与细胞工程。
基金资助:
教育部跨世纪人才基金资助。

PRELIMINARY STUDY ON HYBRID CHROMOSOME COMPOSITION AND RELATIONSHIP IN SYMMETRIC HYBRIDIZATION BETWEEN TRITICUM AESTIVUM AND INTERGENERIC GRASSES

XING Mei-qing¹, XIA Guang-min², CHEN Hui-min²

1. Shandong Experimental High Middle School, Jinan 250001;
2. Shandong University, Jinan 250100

Received:2000-09-20 Online:2001-03-15 Published:2016-06-14

摘要/Abstract

摘要： 采用PEG 法诱导普通小麦的原生质体与新麦草、无芒雀麦及韦氏雀麦等3 种近缘或远缘属间禾草的原生质体进行对称融合。对融合再生细胞系进行形态比较、同工酶分析及5S rDNA间隔序列差异分析, 以鉴定其杂种性质。对部分杂种细胞系进行了染色体组成的分析, 就体细胞杂种中小染色体及染色体断片的产生与异源核质的互作及亲缘远近的关系等问题进行了讨论。

关键词: 普通小麦, 不同亲缘禾草, 体细胞杂交, 杂种染色体组成

Abstract: The protoplasts of three intergeneric grasses (Psathyrostachys juncea, Bromus inermis and Bromus willdenowii Kunth) were fused with wheat (Triticum aestivum. Jinan 177) protoplasts by PEG method respectively. The fusion products of wheat and grasses were analyzed with morphological inspection, isozyme analysis, 5S rDNA spacer sequence analysis. Parts of hybrid clones were taken for chromosome examination. Different chromosome compositions in calli were found between the fusion combinations of different phylogenetical distance, while microchromosomes or chromosome fragments were always present. The reason for this result and the failure of plant regeneration in this experiment is discussed.

Key words: Triticum aestivum, Intergeneric grasses, Symmetric somatic hybridization, Hybrid chromosome composition

邢梅青, 夏光敏, 陈惠民. 小麦与禾草对称融合中杂种染色体组成与亲缘关系的初步探究[J]. 植物研究, 2001, 21(1): 74-78.

XING Mei-qing, XIA Guang-min, CHEN Hui-min . PRELIMINARY STUDY ON HYBRID CHROMOSOME COMPOSITION AND RELATIONSHIP IN SYMMETRIC HYBRIDIZATION BETWEEN TRITICUM AESTIVUM AND INTERGENERIC GRASSES[J]. Bulletin of Botanical Research, 2001, 21(1): 74-78.

[1]	艾可筠, 朱尧, 侯和胜, 佟少明. 普通小麦TaNAC1基因的表达及在拟南芥中的遗传转化[J]. 植物研究, 2016, 36(6): 886-894.
[2]	张东阳;李集临;张延明*. 黑粒小麦的细胞学及分子生物学鉴定[J]. 植物研究, 2010, 30(3): 332-336.