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植物研究 ›› 1999, Vol. 19 ›› Issue (3): 318-322.

• 论文 • 上一篇    下一篇

药用植物北五味子的组织培养

陈雅君1, 吴秀菊1, 关政华1, 杨永富2   

  1. 1. 东北农业大学, 哈尔滨 150030;
    2. 黑龙江省新林林业局, 165023
  • 收稿日期:1997-03-10 出版日期:1999-09-15 发布日期:2016-06-14

THE TISSUE CULTURE OF DRUG PLANT-CHINESE MAGNOLIAVINE

Chen Ya-jun1, Wu Xiu-ju1, Guan Zheng-hua1, Yang Yong-fu2   

  1. 1. Northeast Agricultural University, Harbin 150030;
    2. Xinlin Forestry Bureau, Heilongjiang Province 165023
  • Received:1997-03-10 Online:1999-09-15 Published:2016-06-14

摘要: 取北五味子带腋芽的嫩茎作外植体,培养于附加不同种类和浓度激素的MS培养基上。在附国加6-BA2.0+ZT0.1 mg/L时,诱导芽的效果最佳;在附加6-BA2.0+NAA0.2+ZT0.1 mg/L时,芽的增殖率最好;在附加NAA0.2 mg/L时,生根效果最佳。当苗高2.0~2.5 cm,根数达3~5条时,开瓶锻炼2天,移栽于消过毒的土壤中, 10天后,成活率达100%。

关键词: 北五味子, 外植体, 组织培养

Abstract: The explants of Chinese magnoliavine tender stems with axillary buds are cultured in MS medium with addition of different concentration and kinds of hormone. The results show that sprouting of axillary bud is best on MS medium with addition of 6-BA2.0+ZT0.1 mg/L;and the multiplication of adventitious bud is best on MS medium with the addition of 6-BA2.0+NAA0.2+ZT0.1 mg/L;and the growth of roots is best on MS medium with addition of NAA0.2 mg/L. Seedlings with growing to 2.0~2.5 cm and 3~5 roots are transplanted to the pots with disinfect soil after 2 days training. 10 days later, the survival rate reaches 100%.

Key words: Explant, Chinese magnoliavine, Tissue culture