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植物研究 ›› 1998, Vol. 18 ›› Issue (1): 73-77.

• 论文 • 上一篇    下一篇

“龙麦15”2+12与5+10亚基近等基因系面粉品质差异的研究

张延滨1, 肖志敏1, 祁适雨1, 兰静2, 赵乃新2   

  1. 1. 黑龙江省农业科学院育种所, 哈尔滨 150086;
    2. 农业部谷物及制品质量检测中心, 哈尔滨 150086
  • 收稿日期:1997-01-10 出版日期:1998-03-15 发布日期:2016-06-14
  • 基金资助:
    黑龙江省自然科学基金及黑龙江省农业科学院计划项目资助

FLOUR QUALITY OF NEAR-ISOGENIC LINES OF HMW GLUTENIN SUBUNITS 2+12 AND 5+10 IN CULTIVAR LONGMAI 15

Zhang Yan-bin1, Xiao Zhi-min1, Qi Shi-yu1, Lan Jing2, Zhao Nai-xin2   

  1. 1. Crop Breeding Institute, Heilongjiang Academy of Agricultural Science, Harbin 150086;
    2. Cereal and It's Products Quality Supervisory Inspection and Test Center of Ministry of Agri-culture, Harbin 150086
  • Received:1997-01-10 Online:1998-03-15 Published:2016-06-14

摘要: 通过对小麦栽培品种龙麦15中的同形小种进行SDS-PAGE和A-PA GE的分析,在国内首次获得了一对Glu-D1位点高分子量麦谷蛋白亚基分别为2+12和5+10的近等基因系。对该近等基因系面粉品质的分析表明,带有5+10亚基的龙麦15比带有2+12亚基的龙麦15的Zeleny沉淀值高12%,沉淀值/湿面筋的比值由1.03提高到1.32。该实验结果证明, 5+10亚基对面粉品质贡献确实优于2+12亚基。

关键词: 小麦, 近等基因系, 麦谷蛋白亚基, 面粉品质

Abstract: Near-isogenic lines of HMW glutenin subunits 2+12 and 5+10 of cultivar Longmai 15 were obtained by analysis of morphology, SDS-PAGE and A-PAGE.The Zeleny sedimentation of Longmai 15 with 5+10 subunits is 4.5ml(12%) higher than Longmai 15 with 2+12 subunits.Ratio of Zeleny sedimentation and wet gluten is increased from 1.08 to 1.32.The results indicated that 5+10 subunits is better than 2+12 subunits in flour quality.

Key words: Wheat, near-isogenic line, glutenin subunits, flour quality