欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2009, Vol. 29 ›› Issue (2): 204-208.doi: 10.7525/j.issn.1673-5102.2009.02.014

• 论文 • 上一篇    下一篇

He-Ne激光对增强UV-B辐射小麦幼苗叶绿体的影响

郭君瑜;张琴;韩榕*   

  1. (山西师范大学生命科学学院,临汾 041004)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 韩榕
  • 基金资助:
     

Effects of He-Ne Laser on Chloroplast of Wheat Seedling Exposed to Enhanced UV-B Radiation

GUO Jun-Yu;ZHANG Qin;HAN Rong*   

  1. (College of Life Science,Shanxi Normal University,Linfen 041004)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: HAN Rong
  • Supported by:
     

摘要: 对“晋麦8号”小麦幼苗分别采用5 mW·mm-2 He-Ne激光辐照、10.08 kJ·m-2·d-1增强UV-B辐射及二者组合进行处理,研究各处理组小麦幼苗叶绿体膜透性、叶绿体蛋白质含量以及叶绿体偶联因子CF-1的ATP酶活性、希尔反应的活性变化。结果表明:UV-B辐射后小麦幼苗叶绿体膜透性增加,叶绿体蛋白质含量有一定的下降,而ATP酶活性、希尔反应的活性均受到抑制。经过He-Ne激光辐照可使叶绿体膜透性降低、叶绿体蛋白质含量有一定的升高,同时ATP酶活性、希尔反应的活性也受到部分激活。这些变化说明增强UV-B辐射引起小麦幼苗叶绿体损伤,而一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗光合系统的损伤。

关键词: He-Ne激光, UV-B辐射, 小麦, 叶绿体, 光合系统

Abstract: The seedling of wheat (jinmai8) were exposed to He-Ne laser (5 mW·mm-2), enhanced UV-B radiation (10.08 kJ·m-2·d-1) and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation. The measurement permeability of chloroplast membrane,chloroplast protein content,Hill reaction activity and ATPase activity of wheat seedlings. Result enunciation: the chloroplast membrane permeability has increased after UV-B radiated wheat seedlings,chloroplast protein content went down, Hill reaction activity and ATPase activity were restrained. The He-Ne laser can make chloroplast membrane permeability fall,chloroplast protein content go up certainly. Hill reaction activity and ATPase activity were also partially activated.These variety explain enhanced UV-B radiation cause damage chloroplast of wheat seedling, but certain the He-Ne laser partially repair UV-B damage the system of photosynthesis on wheat seedling.

Key words: He-Ne laser, UV-B, Wheat, Chloroplast, The system of photosynthesis

中图分类号: