欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2005, Vol. 25 ›› Issue (1): 49-52.doi: 10.7525/j.issn.1673-5102.2005.01.017

• 论文 • 上一篇    下一篇

影响小麦成熟胚培养及植株再生因素的研究

李新玲1,2, 曲敏3, 闫玉清1, 徐香玲1   

  1. 1. 哈尔滨师范大学生命与环境科学学院, 哈尔滨 150080;
    2. 东北林业大学林木遗传育种教研室, 哈尔滨 150040;
    3. 哈尔滨商业大学食品工程学院生物系, 哈尔滨 150010
  • 收稿日期:2004-11-02 出版日期:2005-03-15 发布日期:2016-06-14
  • 通讯作者: 徐香玲 E-mail:xxl8761@126.com
  • 作者简介:李新玲(1975-), 女, 博士, 助教, 主要从事植物遗传育种研究工作。
  • 基金资助:
    省科技厅攻关课题(GCO4B112)

Study on the factors of wheat mature embryos culture and plant regeneration

LI Xin-Ling1,2, QUMin3, YAN Yu-Qing1, XU Xiang-Ling1   

  1. 1. School of Life and Environmental Science, Harbin Normal University, Harbin 150080;
    2. Teaching and Research Section of Forestry Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    3. Biology department of Food Engineering College, Harbin University of Commerce, Harbin 150010
  • Received:2004-11-02 Online:2005-03-15 Published:2016-06-14

摘要: 对3个不同栽培品种小麦的成熟胚进行离体培养,研究影响小麦愈伤组织诱导和植株再生的一些因素。结果表明,东农7742的苗分化率明显高于龙麦9814和龙麦26;高浓度的玉米素可明显提高芽的分化率;附加低浓度NAA的1/2 MS培养基可有效促进生根。可见,基因型对小麦愈伤组织的分化有很大的影响,附加一定的外源激素有利于提高植株的再生频率。

关键词: 小麦, 成熟胚, 愈伤组织, 植株再生

Abstract: To culture wheat mature embryos from three cultivars in vitro and study the factors of wheat callus induction and plant regeneration. The result of research shows bud differentiation rate of dongnong 7742 is much higher than longmai 9814 and longmai 26. ZT with high concentration could increase the rate of bud differentiation. The bud formation could be stimulated by half amount of culture medium togetherwith NAA of low concentration. So the genotypes have great influence on wheat callus differentiation and to add certain amount of hormone could increase the rate of plant regeneration.

Key words: wheat, mature embryos, callus, plant regeneration