Simultaneous Determination of 4 Flavonoids in Extracts of Phyllostachys pubescens Leaves by HPLC
ZHANG Xue-Ke;MA Chun-Hui;YANG Lei*;MENG Qing-Huan;WANG Hong-Zheng;ZU Yuan-Gang
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In this study, 4 flavonoids in Phyllostachys pubescens extracts i.e. orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were simultaneously detected. The HPLC detected conditions were as follows: Kromasil-C18 column (5 μm, 4.6 mm×250 mm, Akzol Nobel, Sweden), mobile phase was tetrahydrofuran∶acetonitrile∶methanol∶0.5% aqueous acetic acid (10.3∶1∶0.7∶88), isocratic elution, the detection wavelength was 360 nm, column temperature was 25℃, and 1.0 mL·min-1 flow velocity. The average recoveries of orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were 98.5%, 98.1%, 99.1%, and 98.7%, respectively. The relative standard deviations were 0.8%, 0.7%, 0.8% and 0.6%(n=5), respectively. This HPLC method has been proved to be a method with high efficiency, rapid analysis, high sensitivity and good reproducibility for detection of flavonoids in P.pubescens extracts.