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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (3): 423-427.doi: 10.7525/j.issn.1673-5102.2014.03.021

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HPLC Fingerprints of 12 Taraxacum from Jilin Provinc

LI Jing-Hua;ZHONG Jia-Jia;LIU Yu-Qin;ZHANG Yu-Hong*   

  1. 1.Department of Pharmacy,Jilin Medical College,Jilin 132013;2.Potrochina Jili Petrochemical Company of Jilin City,Jilin 132022;3.Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Supported by:

Abstract: To explore the characteristics of HPLC fingerprints of Taraxacum in Jilin province and provide a theoretical reference for the quality control of dandelion herbs, the HPLC fingerprints of Taraxacum in Jilin province were compared. HPLC analysis was carried out in Agilent Extend-C18(4.6 mm×250 mm, 5 μm) column with a mobile phase of methanol and 0.5% acetic acid water at the flow of 1.000 mL/min, the detection wavelength was 323 nm, the temperature was 35℃, the injection volume was 20 μL. The established fingerprint of herba Taraxaci was used to determine 12 batches of samples from different sources. Nine peaks were identified as the character fingerprints through similarity analysis and 3 compounds among them were determined as chlorogenic acid, caffeic acid and flavonoids by HPLC. Quantitative results showed that the average contents of three kinds of ingredients were 0.027%, 0.026% and 0.128%, respectively. The method has good precision, reproducibility and stability, and can be used as the quality control of Herba taraxaci in Jilin Province.

Key words: Taraxacum, HPLC, fingerprint

CLC Number: