Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2016.03.012

Abstract

Abstract: Zinc finger proteins constitute a large family of transcription factors and numerous members of them play diverse roles in many biological processes, including plant growth, development and stress responses. Based on their structural and functional diversities they were classified into nine types including C₂H₂, C₈, C₆, C₃HC₄, C₂HC, C₂HC₅, C₄, CCCH and C₄HC₃. We studied the genes in Betula platyphylla database by the sequences and homology comparison of genes encoding zinc finger proteins involving in stress responses from other organisms. A total of ten genes (designated as BpZFP1 to BpZFP10), of which four were C₂H₂ type, five were C₃HC₄-RING type, one was CCCH type were identified. By quantitative RT-PCR assay, we analyzed the expression patterns of BpZFPs in roots. BpZFP2, BpZFP3, BpZFP4, BpZFP5 and BpZFP8 were induced under high-salt and drought treatment significantly indicating their involvement in stress response in Birch.

Key words: Betula platyphylla, abiotic stress, zinc finger transcription factor, expression analysis

CLC Number:

S792.153

WANG Yu-Hang, QIN Lin-Lin, LI Li. Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla[J]. Bulletin of Botanical Research, 2016, 36(3): 395-400.

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