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Bulletin of Botanical Research ›› 2000, Vol. 20 ›› Issue (2): 201-206.

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RAPD ANALYSIS ON GENETIC VARIATION OF PINUS KORAIENSIS IN LIANG SHUI NATIONAL NATURAL RESERVE

ZHANG Heng-qing1, AN Li-jia3, ZU Yuan-gang2   

  1. 1. Department of Biology LiaoNing Normal University Dalian 1 16029;
    2. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040;
    3. Department of Bioengineering, Dalian 116012
  • Received:1999-07-29 Online:2000-06-15 Published:2016-06-14

Abstract: The genetic variation and the regulation of genetic diversity distribution of Pinus koraiensis were studied by random amplified polymorphic DNA (RAPD) analysis. 72 individuals come from 8 plots in Liang Shui natural reserve were amplified by PCR reaction. A total of 96 loci were obtained from 1 6 random primers, among which 55 loci were polymorphic. In species level, the percentage of polymorphic loci is P=58.51%, Nei's gene diversity is H=0.2868 and Shannon's information index is I=0.3654. The degree of genetic differentiation among 8 plots Gst=0.0753. According to the characterized of every plot, relation ship between the level of genetic variation and character of population habitat were discussed.

Key words: Pinus koraiensis, RAPD, genetic variation, genetic diversity