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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (2): 294-301.doi: 10.7525/j.issn.1673-5102.2021.02.017

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Cloning and Expression Analysis of Two Key Genes of Jasmonic Acid Synthesis in Response to Endophytic Infection from Rehmannia glutinosa

Shu-Ping PENG1, Cheng-Ming DONG1,2, Yun-Hao ZHU1,2()   

  1. 1.School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046
    2.Co -construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P. R. China,Zhengzhou 450046
  • Received:2020-01-14 Online:2021-03-20 Published:2021-01-05
  • Contact: Yun-Hao ZHU E-mail:guxinghan123@163.com
  • About author:Peng Shu-Ping(1996—),female,postgraduate,molecular biology of medicinal plants research.
  • Supported by:
    National Natural Science Foundation of China(81603232);National key research and development plan(2017YFC1702800)


Allene oxide synthase(AOS) and 12-oxophytodienoate reductase(OPR) were the key genes of jasmonic acid biosynthesis in the plants. The genes of AOS and OPR responding to endophytic fungus infection were screened from the interaction transcriptome of Rehmannia glutinosa with endophytic fungus GG22. Specific primers were designed to the open reading frame of RgAOS and RgOPR. Bioinformatics analysis of the sequences and theirs encoded product were performed, and real-time fluorescent quantitative PCR was used to analyze the expression patterns of RgAOS and RgOPR in different tissues infection by endophytic fungus GG22. The open reading frame of RgAOS was 1 626 bp, encoding 541 amino acids with a molecular weight of 60.2 kD. The open reading frame of RgOPR was 1 197 bp, encoding 398 amino acids with a molecular weight of 44.07 kD. QPCR analysis showed that the expression of RgAOS was the highest in roots, the lowest in flowers, and the expression of RgOPR was higher in flowers and leaves, respectively. Endophytic fungus GG22 induced the expression of RgAOS and RgOPR in R.glutinosa. The RgAOS and RgOPR were successfully cloned from R.glutinosa, laying the foundation for further study on the biological activity of Jasmonic acid substances in R.glutinosa and claiming the molecular mechanism of endophytic fungi in the regulation of secondary metabolites of R.glutinosa.

Key words: allene oxide synthase, 12-oxophytodienoate reductase, bioinformatics analysis, expression analysis, Rehmannia glutinosa

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