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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (2): 281-294.doi: 10.7525/j.issn.1673-5102.2021.02.016

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Leaf Phenotype Variation and Heterosis in F1 Progeny of Cross Between Castanea mollissima and C.henryi

Ping-Sheng ZHANG1,2, Xi-Bing JIANG1, Bang-Chu GONG1(), Yang XU1, Jun-sheng LAI3, Cong-Lian WU3   

  1. 1.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400
    2.Nanjing Forest University,Nanjing 210037
    3.Forestry Administration of Qingyuan County,Qingyuan 323800
  • Received:2020-01-06 Online:2021-03-20 Published:2021-01-05
  • Contact: Bang-Chu GONG E-mail:gongbc@126.com
  • About author:ZHANG Ping-Sheng(1994─),male,postgraduate student,major in economic forest breeding and cultivation.
  • Supported by:
    Key Program of the Central Public-Interest Scientific Institution Basal Research Fund(CAFYBB2017ZA004-6);Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(2016C02056-6)


In order to explore the genetic variation and heterosis of leaf traits in cross F1 progeny of Castanea, 14 traits of leaf phenotype and photosynthetic physiology of F1 progeny(including 235 individuals) of 9 combinations (including interspecific and intraspecific cross between C.mollissima and C.henryi) and their parents were studied by using the statistical methods of Nested anova, Pearson correlation analysis and principal component analysis. The results showed: ①The average coefficient of variation of 14 traits ranged from 4.40%-27.76%, indicating that there were abundant genetic diversity in leaf phenotype and photosynthetic physiological traits in F1 hybrid progeny of Castanea. ②The average of phenotypic differentiation coefficient among combination were 32.32%, which showed that the variation within combinations was the main source of variation. ③The mid-parent heterosis rate of 14 traits ranged from -27.16%-90.53%, and the heterosis of different combinations was different. ④The Pearson correlation analysis showed that there were 46 and 13 pairs of correlations reached extremely significant levels(P<0.01) and significant levels(P<0.05), respectively, and 5 leaf phenotype traits, such as leaf length and leaf width, and 4 photosynthetic physiological indexes, such as chlorophyll a and chlorophyll b, had a great influence on each other. ⑤2 crossing combinations ‘Kuili’×YLZ No.15 and ‘Kuili’×YLZ No.1 were finally selected by principal component analysis, and their offspring had the characteristics of large leaf area, high dry matter and photosynthetic pigment content, which can provide the main materials for further screening of excellent offspring.

Key words: Castanea, hybrid offspring, genetic variation, heterosis

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