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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 123-130.doi: 10.7525/j.issn.1673-5102.2019.01.015

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Antisense CCoAOMT Gene Regulates Lignin Biosynthesis in Betula platyphylla

YAO Lian-Mei1, HU Xiao-Qing1, ZHOU Fei2, ZHENG Yao-Qiang1, WANG Guo-Dong1   

  1. 1. College of Biological Sciences and Biotechnology, Northeast Forestry University, Harbin 150040;
    2. Institute of Economic Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150040
  • Received:2018-07-02 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    Project name (number) Special Fund for Basic Scientific research operation Fee of Central University(2572015EA05)

Abstract: Betula platyphylla is an important forestation tree species in northern China, but its high lignin content severely restricts its development and utilization as a papermaking resource plant. In this paper, the full-length ORF sequence of CCoAOMT(Caffeoyl-coenzyme A-3-O-methyltransferase) gene was obtained using RACE technology. The antisense CCoAOMT expression vector was constructed and transformed into B.platyphylla by Agrobacterium infection method. The molecular detection indicated that the CCoAOMT antisense gene had been successfully integrated into B.platyphylla. By semi-quantitative PCR analysis of the transgenic plants, the CCoAOMT gene expression of the transgenic lines was decreased. Compared to the wild type, by the Wiesner staining, the lignin content of the transgenic plants was decreased. Chemical compositions of seven-year-old transgenic and wild-type birch were analyzed. The phenyl alcohol extracts and Klason lignin of transgenic birch were significantly reduced, and the pentosan content was increased. The BpCCoAOMT gene plays an important role in the synthesis of lignin, which lays a foundation for the cultivation of a new species of birch with low lignin content.

Key words: CCoAOMT gene, antisense RNA, birch, lignin, Wiesner stain, transgene

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