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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (5): 779-785.doi: 10.7525/j.issn.1673-5102.2015.05.023

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Microwave-assisted Polysaccharide Extraction of Aralia chinensis L. Root and Composition Determination of Monosaccharide

WANG Yi-Feng;HOU Hong-Hong;JIN Jie;ZHAO Bo;CAO Jia-Hao;LI Xiao-Jiao   

  1. College of Life Sciences,Northwest Normal University,Lanzhou 730070
  • Online:2015-09-15 Published:2015-11-20
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Abstract: We extracted the Aralia chinensis polysaccharide(ACP) from A.chinensis L. root by microwave-assisted technique. With the single factor experiment, we optimized four factors affecting the yield of A.chinensis root including time, temperature, microwave power and solid-liquid ratio by using orthogonal design method. The optimum extracting conditions for polysaccharide yield of 15.189% was under the ratio of solid to liquid 1∶30 (g·mL-1), 40 min, 50℃, and microwave power of 400 W. Polysaccharides were deproteinized by Sevage method after dialysis treatment, and A.chinensis root had infrared spectral scanning polysaccharide characteristic absorption peaks. Ee measured the molecular weight of A.chinensis root polysaccharides by using SEC-HPLC and LLS: Mn=4.8×105 g·mol-1. A.chinensis root hydrolysis was derivatized by gas chromatography-mass spectrometry (GC-MS) analysis, and the polysaccharide of A.chinensis were composed of D-arabinose, D-glucose and D-galactose, with the molar ratio of 4.67∶76.44∶18.18.

Key words: microwave-assisted extraction, Aralia chinensis L. polysaccharide, orthogonal test, molecular weight, monosaccharide composition

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