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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (1): 146-149.doi: 10.7525/j.issn.1673-5102.2015.01.022

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A Micro-propagation Technique of Corylus heterophylla×C.avellana


  1. Forestry Research Institute of Heilongjiang Province,Harbin 150081
  • Online:2015-01-15 Published:2015-03-11
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Abstract: In cold resistance appraisal of fine varieties hybrid hazelnut leaves, stems, axillary bud cotyledons, we studied various treatments on cold resistance hybrid hazelnut micro-propagation system with different culture medium, hormones and contents. By using ten tissue cultures of a fine varieties hardiness of F1 hybrid varieties, we selected the effect of three superior varieties 84 226, 84 310, 84 253. By using three culture medium of DKW, MS and WPM, WPM was more suitable for cold resistance hazelnut callus induction medium, and MS was the most suitable medium culture propagation. The basic training callus induction based formulations was cultured in WPM+6-BA 2.0 mg·L-1+NAA 1.5 mg·L-1, and axillary bud propagation was in MS in good condition with the hormone of 6-BA 1.5 mg·L-1+NAA 0.1 mg·L-1. Our study can be used in cold resistance of hybrid hazelnut low coefficient, and can provide a theoretical basis for further industrial production plantlets.

Key words: Corylus heterophylla×, C.avellana, micro-propagation

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