Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (3): 369-372.doi: 10.7525/j.issn.1673-5102.2010.03.021

Previous Articles     Next Articles

Optimization of Extration of 7-xylosyl-10-deacetylpaclitaxel from Taxus cuspidata

ZHANG Yu;JIANG Shou-Gang;ZU Yuan-Gang*;WANG Zhuo;FU Yu-Jie   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: ZU Yuan-Gang
  • Supported by:

Abstract: In an effort to search for natural products from Taxus cuspidata with improved pharmacological features compared to paclitaxel,7-xylosyl-10-deacetylpaclitaxel has been isolated and identified by our group. The objective of the present study was to optimize and establish the extraction process of 7-xylosyl-10-deacetylpaclitaxel from T.cuspidata. RP-HPLC mothod was applied to determine the content of 7-xylosyl-10-deacetylpaclitaxel. The orthogonal test was adopted to examine the effects of the 4 factors including ethanol concentration, ethanol volume, number of extraction cycles and extraction time. Ethanol concentration and ethanol volume were essential factors influencing the extraction of 7-xylosyl-10-deacetylpaclitaxel. High concentration and amount of ethanol facilitated the extraction. Number or extraction cycles and extraction time were also important. Single-factor test indicated, 2 h and 2 cycles were the best. The optimum extractive condition are N9(A3B3C2D1) and N8(A3B2C1D3).

Key words: 7-xylosyl-10-deacetylpaclitaxel, HPLC, separation and purification, identification, orthogonal test

CLC Number: