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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 559-564.doi: 10.7525/j.issn.1673-5102.2009.05.010

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Toxic Effects of Cr6+ on Chlorophyll Fluorescence Parameters,Antioxidant Systems and Ultrastructure of Potamogeton crispus

CHEN Hui;SHI Guo-Xin*;XU Qin-Song;Wang Hong-Xia;Yang Hai-Yan;PAN Qiu-Hong   

  1. (College of Life Science,Nanjing Normal University,Jiangsu Key Lab of Biodiversity and Biotechnology,Nanjing210046)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: SHI Guo-Xin
  • Supported by:

Abstract: The toxic effects of Cr6+(0,1,10,30,50 mg·L-1) on chlorophyll, chlorophyll fluorescence parameters, antioxidant systems, soluble protein, lipid peroxidation, soluble sugar, active oxygen generation and ultrastructure changes in leaf of Potamogeton crispus were studied. The results showed that with increasing concentration of Cr6+,the contents of chlorophyll, chlorophyll a, chlorophyll b and chlorophyll a/b decreased gradually, and Fo enhanced progressively up to 10 mg·L-1 Cr6+ and followed by a decline at higher dose. Fv/Fm, Fm and Fv/Fo decreased gradually. Soluble protein content and ascorbic acid(AsA) content enhanced and followed by a decline, glutathione(GSH) content decreased gradually.The activity of superoxide dismutase(SOD) decreased gradually, and activities of peroxidase(POD), catalase(CAT), ascorbate peroxidase(APX), glutathione reductase(GR) increased first and then decreased. While O2—·, MDA and soluble sugar content enhanced and followed by a decline. Transmission electron microscope observation indicated that Cr6+ also imposed injury action on ultrastructure of leaf cells, swelling of chloroplast and breakage of chloroplast envelope, decreasing of cristae quantity and vacuolization of mitochondria were observed. The conclusion could be reached that the plant death was resulted from destruction under structure foundation of physiological function, disorder of physiological metabolism.

Key words: Potamogeton crispus, chlorophyll fluorescence, Cr6+ Stress, antioxidant System, ultrastructure

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