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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (3): 302-307.doi: 10.7525/j.issn.1673-5102.2006.03.013

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Construction and analysis of Tamarix albiflonum suppression subtractive hybridization library under osmotic stress

DONG Yu-Zhi;YANG Chuan-Ping*;WANG Yu-Cheng;ZHANG Dao-Yuan   

  1. 1.School of Forestry,Northeast Forestry University,Harbin 150040 2.Forestry College of Xinjiang Agricultural University,Urumqi 830052 3.Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences,Urumqi 830011
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-20 Published:2006-05-20
  • Contact: YANG Chuan-Ping
  • Supported by:

Abstract: A suppression subtractive hybridization library of Tamarix albiflonum was constructed by using the osmosis treated leaves as tester and leaves grown under normal conditions as driver. The PCR results of recombinant plasmid showed that the inserts were mainly 250~650 bp. After alignment with NCBI database, 23 osmosis stress related genes, such as proline transport protein, calciumdependent protein kinase, leucine zipper-containing protein, translation initiation factor-like protein,which responsible for osmotic regulating, signal transduction, gene regulating, antioxidation and metabolism were detected.

Key words: Tamarix albiflonum, osmotic stress, suppression subtractive hybridization, expressed sequence tags

CLC Number: