Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan

doi:10.7525/j.issn.1673-5102.2012.02.016

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (2): 222-226.doi: 10.7525/j.issn.1673-5102.2012.02.016

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Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan

WU Yun-Yun;TIAN Lu;ZHU Yong-Ping;HE Feng-Mei*

1.College of Horticulture and Landscape,Yunnan Agricultural University,Kunming　650201；2.College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming　650201

Received:1900-01-01 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20
Contact: HE Feng-Mei
Supported by:

Abstract

Abstract: In order to explore the molecular mechanism of orchid labellum formation, the suppression subtractive hybridization (SSH) cDNA library was constructed with petals and labellum of Cymbidium lianpan in this study. One hundred and forty-seven randomly selected clones of positive library were sequenced, assembled and spliced, 37 unique sequences were found, containing 25 single sequences and 12 spliced sequences. After homology comparison, these ESTs were classified as protein folding, binding, unknown function, stress response, photosynthesis, transcription regulation, signal transduction, biosynthesis, metabolism, MADS and other 10 categories. Through the analysis of phylogenetic tree about MADS-box genes, we can initially speculate that MADS-Contig1 and MADS-Contig2 belong to A class SQUA-like subfamily genes.

Key words: Cymbidium lianpan, petals, labellum, suppression subtractive hybridization, MADS genes

CLC Number:

S682.1

WU Yun-Yun;TIAN Lu;ZHU Yong-Ping;HE Feng-Mei*. Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan[J]. Bulletin of Botanical Research, 2012, 32(2): 222-226.

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Construction of SSH cDNA Library and the Corresponding EST Analysis in Petals and Labellum of Cymbidium lianpan

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