拟南芥转录因子Ethylene-insensitive3（EIN3）抑制花青素的合成

doi:10.7525/j.issn.1673-5102.2018.01.018

摘要/Abstract

摘要： Ethylene-insensitive3（EIN3）和 EIN3-like1（EIL1）蛋白是乙烯信号转导途径中一类重要的核转录因子。花青素是植物体中的一类水溶性天然色素，在植物的许多生理过程中起重要作用。本研究以拟南芥双突变体ein3-1eil1-3为研究材料，通过RT-PCR技术确定了拟南芥双突变体ein3-1eil1-3中EIN3和EIL1基因均已被敲除，单突变体ein3-1中的EIN3基因被敲除。通过肉眼定性观察发现突变体ein3-1eil1-3的种子和叶片内均呈紫色。通过紫外分光光度计定量分析发现，花青素积累量也明显比突变体ein3-1和野生型多。通过GUS染色发现EIN3启动子主要在花、柱头、成熟花粉、种子胚和果荚等组织中有较强的表达。这与突变体ein3-1eil1-3的种子和叶片内均呈紫色并花青素含量增高一致。因此，拟南芥转录因子EIN3可能与EIL1共同参与抑制花青素的合成。

关键词: Ethylene-insensitive3（EIN3）, Ethylene-insensitive3-like 1（EIL1）, 花青素, 拟南芥

Abstract: Ethylene-insensitive3(EIN3) and EIN3-like1(EIL1) proteins are important nuclear transcription factors in ethylene signal transduction pathways in higher plants. In plants, anthocyanins are one of the water-soluble natural pigments, and they play an important role in the growth and development of plants. We used the Arabidopsis thaliana double mutant ein3-1eil1-3 as the research material. Firstly, we identified the mutants of ein3-1eil1-3 and ein3-1 by RT-PCR. The direct observation indicated that the seeds and leaves of the ein3-1eil1-3 were purple. By ultraviolet spectrophotometer analysis, the anthocyanin accumulation in ein3-1eil1-3 mutant was significantly higher than that in the ein3-1 mutant and wild-type. GUS staining showed that EIN3 was mainly expressed in flowers, stigma, mature pollen, seed embryo and siliques, which was consistent with that anthocyanin accumulation of seeds and leaves was high in mutant ein3-1eil1-3. Thus, A.thaliana transcription factor EIN3 as well as EIL1 is involved in the suppression of the anthocyanin synthesis.

Key words: ethylene-insensitive3(EIN3), ethylene-insensitive3-like 1(EIL1), anthocyanins, Arabidopsis thaliana

中图分类号:

Q786

徐梦珂, 李丹, 孟来生, 蒋继宏. 拟南芥转录因子Ethylene-insensitive3（EIN3）抑制花青素的合成[J]. 植物研究, 2018, 38(1): 148-154.

XU Meng-Ke, LI Dan, MENG Lai-Sheng, JIANG Ji-Hong. Arabidopsis thaliana Transcription Factor Ethylene-insensitive3(EIN3) Inhibits the Synthesis of Anthocyanins[J]. Bulletin of Botanical Research, 2018, 38(1): 148-154.

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