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植物研究 ›› 2017, Vol. 37 ›› Issue (4): 535-541.doi: 10.7525/j.issn.1673-5102.2017.04.008

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5个芍药属伊藤杂种的倍性鉴定及核型分析

杨柳慧1,2,3, 张建军1,2,3, 王琪1,2,3, 朱炜1,2,3, 张滕1,2,3, 于晓南1,2,3   

  1. 1. 北京林业大学园林学院, 北京 100083;
    2. 花卉种质资源创新与分子育种北京重点实验室, 北京 100083;
    3. 国家花卉工程技术研究中心, 北京 100083
  • 收稿日期:2017-02-21 出版日期:2017-07-15 发布日期:2017-07-22
  • 通讯作者: 于晓南,E-mail:yuxiaonan626@126.com E-mail:yuxiaonan626@126.com
  • 作者简介:杨柳慧(1992-),女,硕士研究生,主要从事芍药资源与育种方面的研究。
  • 基金资助:
    受北京市教育委员会科学研究与研究生培养共建项目资助(BLCXY201615);国家自然科学基金(31400591)

Ploidy Identification and Karyotype Analysis of Five Itoh Hybrid Peonies

YANG Liu-Hui1,2,3, ZHANG Jian-Jun1,2,3, WANG Qi1,2,3, ZHU Wei1,2,3, ZHANG Teng1,2,3, YU Xiao-Nan1,2,3   

  1. 1. College of Landscape Architecture, Beijing Forestry University, Beijing 100083;
    2. Beijing Key Laboratory for Flower Germplasm Innovation and Molecular Breeding, Beijing 100083;
    3. National Engineer Research Center for Floriculture, Beijing 100083
  • Received:2017-02-21 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    This work was financially supported by Graduate Training and Development Program of Beijing Municipal Commission of Education(BLCXY201615);National Natural Science Foundation of China(31400591)

摘要: 采用常规压片法,对5个芍药属伊藤杂种进行了核型分析。结果表明:5个芍药属伊藤杂种的品种均为三倍体(2n=3x=15),核型不对称系数为61.02%~62.41%,核型类型均属于2A型,所有品种的核型均由中部(m)、近中部(sm)和近端部(st)的着丝粒染色体组成。本研究首次摸清了芍药属伊藤杂种的核型背景,为品种改良提供了细胞学资料。

关键词: 伊藤杂种, 三倍体, 核型参数

Abstract: We studied the karyotypes of five Itoh hybrid peonies by using squashing method. Five Itoh hybrid peonies were triploid(2n=3x=15). Asymmetry index ranged in 61.02%-62.41%, and the karyotypes of these cultivars were 2A. The karyotypes of the tested Itoh hybrid peonies were mainly made of median region, submedian region and subterminal region, without satellite. Our result provided cytological information and polyploidy resources for breeding research for the first time.

Key words: Itoh hybrid peonies, triploid, karyotype chromosomes

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