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植物研究 ›› 2017, Vol. 37 ›› Issue (2): 211-215.doi: 10.7525/j.issn.1673-5102.2017.02.008

• 论文 • 上一篇    下一篇

盐胁迫诱导盐穗木Rev1、Rev3基因的表达分析

杜驰1,2, 张冀1,2, 张富春1,2   

  1. 1. 新疆大学生命科学与技术学院, 乌鲁木齐 830046;
    2. 新疆生物资源基因工程重点实验室, 乌鲁木齐 830046
  • 收稿日期:2016-11-03 出版日期:2017-03-15 发布日期:2017-03-11
  • 通讯作者: 张富春,E-mail:zfcxju@xju.edu.cn E-mail:zfcxju@xju.edu.cn
  • 作者简介:杜驰(1990-),女,硕士研究生,主要从事植物分子生物学研究。
  • 基金资助:
    新疆重点实验室专项资金资助项目(2014KL001)

Expression Analysis of Rev1 and Rev3 of Halostachys caspica under Salt Stress

DU Chi1,2, ZHANG Ji1,2, ZHANG Fu-Chun1,2   

  1. 1. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
    2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046
  • Received:2016-11-03 Online:2017-03-15 Published:2017-03-11
  • Supported by:
    Supported by Special Funds of Xinjiang Key Laboratory(2014KL001)

摘要: 根据盐穗木盐胁迫下响应的转录组测序结果,参考盐穗木HcRev1、HcRev3基因的ESTs序列设计荧光定量PCR特异性引物,建立检测盐穗木Revs基因相对表达量的荧光定量PCR方法,分析Rev1和Rev3基因在盐穗木不同浓度盐胁迫处理不同时间的转录水平。结果表明,HcRev1、HcRev3基因具有相似的表达模式,在100 mmol·L-1 NaCl低盐胁迫下表达稳定,在300、500、700 mmol·L-1 NaCl胁迫下,随胁迫浓度增高、胁迫时间延长,表达量升高。其中HcRev1在700 mmol·L-1 NaCl胁迫14 d后达到峰值,是对照组的4.63倍。HcRev3基因在300 mmol·L-1 NaCl胁迫14 d时,表达量迅速升高,是对照组的15.55倍,表达差异极显著。研究结果说明HcRev1、HcRev3基因都受盐胁迫诱导表达,提示HcRev1、HcRev3基因虽然表达量存在差异,但在盐胁迫过程中参与了DNA损伤修复。研究有助于阐明Rev1、Rev3基因在DNA损伤修复和植物耐盐性间的调控功能作用。

关键词: 盐穗木, HcRev1基因, HcRev3基因, 盐胁迫, 表达分析

Abstract: According to the transcriptome of Halostachys caspica under salt stress, the real-time PCR primers designed about Rev1 and Rev3 were referred to the ESTs of transcriptome sequencing. We established a method for detecting of mRNA transcription of Revs by Real-time PCR, and the transcription level of Revs in different concentration of NaCl present. Two genes have similar expression pattern, the expression levels remain stable under 100 mmol·L-1 NaCl treatment. As the concentration of NaCl was increased and the stress time was prolonged, the genes relative expression levels were also gradually increased. HcRev1 gene in 700 mmol·L-1 NaCl stress for 14 d was rapidly up-regulated and reached the peak level, which was 4.63-fold of the control. HcRev3 gene in 300 mmol·L-1 NaCl stress for 14 d was 15.55-fold of the control with significant differences. Both HcRev1 and HcRev3 genes were induced by salt stress, although there were different expression levels of HcRev1 and HcRev3 genes, which were involved in the DNA damage repair during salt stress.Our study could be used to clarify the regulatory function of HcRev1 and HcRev3 genes between DNA damage-repair and salt resistance in plants.

Key words: Halostachys caspica, HcRev1 gene, HcRev3 gene, salt stress, expression analysis

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