固相微萃取—气相色谱—质谱联用分析蓼实挥发性成分

doi:10.7525/j.issn.1673-5102.2008.06.027

植物研究 ›› 2008, Vol. 28 ›› Issue (6): 770-774.doi: 10.7525/j.issn.1673-5102.2008.06.027

• 论文 • 上一篇

固相微萃取—气相色谱—质谱联用分析蓼实挥发性成分

陈艳¹;薛小娟²;朱宏^1*

(1.华南师范大学化学与环境学院，广州 510006) (2.中国科学院亚热带农业生态研究所，长沙 410125)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-20 发布日期:2008-11-20
通讯作者: 朱宏
基金资助:

Analysis of the Volatile Constituents from Seeds of Polygonum orientale by Gas Chromatography-Mass Spectrometry Coupled with Solid-phase Microextraction

CHEN Yan;XUE Xiao-Juan;ZHU Hong*

(1.School of Chemistry and Environment,South China Normal University,Guangzhou　510006) (2.Institute of Subtropical Agriculture,Chinese Academy of Science,Changsha　410125)

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20
Contact: ZHU Hong
Supported by:

摘要/Abstract

摘要： 察了萃取样品温度、萃取纤维吸附时间等因素对于固相微萃取蓼实挥发性成分的影响，确定较佳的实验条件为：萃取样品温度60℃，萃取纤维吸附时间60 min，脱附温度250℃，脱附时间5 min。用气相色谱—质谱联用技术测定上述条件所得蓼实挥发性化学成分，并鉴定出其中43种，占总峰面积的76.73%。其中含量较高的物质有：罗汉柏烯 (6.99%),丁香烯 (5.59%)，2,5,5,8 a-四甲基-6,7,8,8a四氢-5H-萘-1-酮(5.52%),α-丁香烯(4.29%)，1,2,4a,5,6,8a-六氢-4,7-二甲基-1-异丙基萘(4.04%)，环氧石竹烯(3.60%),α-香附酮(3.54%)，4,5,5a,6,6a,6b-6氢-4,4,6b-三甲基-2-乙烯基-2H-环丙香豆酮(3.54%)，香叶基丙酮(3.48%)。

关键词: 蓼实, 挥发性成分, 固相微萃取, 气相色谱&mdash, 质谱联用

Abstract: Effect of sample temperature and adsorption time on the SPME(solid phase microextraction) treatment of seeds of Polygonum orientale L. were investigated. The optimal extraction conditions were absorption at 60℃ for 60 min and desorption at 250℃ for 5 min. Samples of seeds of P. orientale L. were analyzed by GC-MS coupled with SPME. Through comparison with mass spectra database，forty-tree components were identified，covering 76.73% of the total peak areas in the GC-MS total ion current chromatography. The characteristic components were Thujopsene(6.99%),Caryophyllene5.59%),2,5,5,8a-Tetramethyl-6,7,8,8a-tetrahydro-5H-naphthalen-1-one(5.52%),α-Caryophyllene(4.29%),1,2,4a,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-Naphthalene(4.04%),Caryophylleneoxide(3.60%),α-Cyperone(3.54%),4,5,5a,6,6a,6b-hexahydro-4,4,6b-trimethyl-2-(1methylethenyl)-2H-Cyclopropa-benzofura n(3.54%),Geranylacetone(3.48%).

Key words: seeds of Polygonum orientale L., volatile constituents, solid phase microextraction, gas chromatography-mass spectrometry(GC-MS)

中图分类号:

O657.7

陈艳;薛小娟;朱宏*. 固相微萃取—气相色谱—质谱联用分析蓼实挥发性成分[J]. 植物研究, 2008, 28(6): 770-774.

CHEN Yan;XUE Xiao-Juan;ZHU Hong*. Analysis of the Volatile Constituents from Seeds of Polygonum orientale by Gas Chromatography-Mass Spectrometry Coupled with Solid-phase Microextraction[J]. Bulletin of Botanical Research, 2008, 28(6): 770-774.

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固相微萃取—气相色谱—质谱联用分析蓼实挥发性成分

Analysis of the Volatile Constituents from Seeds of Polygonum orientale by Gas Chromatography-Mass Spectrometry Coupled with Solid-phase Microextraction

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