HPLC法测定甘薯叶片中的叶黄素

doi:10.7525/j.issn.1673-5102.2008.06.026

植物研究 ›› 2008, Vol. 28 ›› Issue (6): 767-769.doi: 10.7525/j.issn.1673-5102.2008.06.026

HPLC法测定甘薯叶片中的叶黄素

石晋¹;栾雨时^1,2*;王静云¹;田俊平¹

(1.大连理工大学环境与生命学院，大连 116023) (2.农业部寒地作物生理生态重点开放实验室，哈尔滨 150030)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-20 发布日期:2008-11-20
通讯作者: 栾雨时
基金资助:

Determination of Lutein in Leaves of Sweet Potato by HPLC

SHI Jin;LUAN Yu-Shi;*;WANG Jing-Yun;TIAN Jun-Ping

(1.School of Environmental and Biological Science & Technology,Dalian University of Technology,Dalian　116023)(2.Key Laboratory of Physiology and Ecology of Cold Region Crops,Ministry of Agriculture,Harbin　150030)

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20
Contact: LUAN Yu-Shi
Supported by:

摘要/Abstract

摘要： 建立甘薯叶片中有效成分叶黄素含量的高效液相色谱测定方法，以寻求含量高的甘薯品种。采用Waters SunFire^TM C₁₈(150 mm×4.6 mm,5 μm )色谱柱；以甲醇—水为95:5(V/V)为流动相，流速为0.8 mL·min^-1；检测波长为445 nm；外标法定量。此色谱条件下，叶黄素含量在5~100 μg·mL^-1范围内时，与峰面积呈线性关系；样品平均回收率为99.5%；相对标准偏差 (RSD)为1.9%(n=5)。该方法灵敏、准确、专属性强，适用于甘薯叶片中叶黄素的测定；大部分供试品种间存在显著差异，其中以苏薯8号的含量最高。

关键词: 叶黄素, 甘薯, HPLC

Abstract: A HPLC method for the determination of lutein in leaves of sweet potato was developed in this research for selecting the breed which has the highest content of lutein. The analysis was carried on a Waters SunFire^TM C₁₈(150 mm×4.6 mm,5 μm) column. The mobile phase was methanol-water (95:5, v/v), the flow-rate was 0.8 mL·min^-1 and wavelength was 445 nm. Linearity was found in the range of 5~100 μg·mL^-1, the average recovery was 99.5%, and the relative standard deviations for lutein was 1.9%(n=5). This method is reliable, accurate and suitable for the determination of lutein in leaves of sweet potato. There were significant differences in the lutein content of most samples tested and among them, Sushu No.8 are the highest.

Key words: lutein, sweet potato, HPLC

中图分类号:

Q946

石晋;栾雨时;*;王静云;田俊平. HPLC法测定甘薯叶片中的叶黄素[J]. 植物研究, 2008, 28(6): 767-769.

SHI Jin;LUAN Yu-Shi;*;WANG Jing-Yun;TIAN Jun-Ping. Determination of Lutein in Leaves of Sweet Potato by HPLC[J]. Bulletin of Botanical Research, 2008, 28(6): 767-769.

[1]	山丹, 屈璐璐, 李晓杰, 马一鸣, 王俊杰. 不同地区草乌种子中生物碱含量的测定与分析[J]. 植物研究, 2020, 40(5): 768-774.
[2]	谈传婷, 宋炜涵, 朱明库, 徐涛, 董婷婷, 李宗芸. 甘薯MADS-box转录因子IbMADS11-Like的克隆及胁迫响应分析[J]. 植物研究, 2017, 37(4): 587-595.
[3]	邓绍立;姜国斌*;任贤;金华;马金龙;邹吉祥. 干旱胁迫下杨树嫩茎质外体内源激素的响应[J]. 植物研究, 2013, 33(6): 690-696.
[4]	孙晓莉;刘帅华;汪雷;李汶罡;赵春建*;祖元刚. 高效液相色谱法同时测定五指山绿茶中5种儿茶素类成分[J]. 植物研究, 2011, 31(3): 377-380.
[5]	张宇;姜守刚;祖元刚*;汪琢;付玉杰. 7-木糖-10-去乙酰基紫杉醇的优化试验研究[J]. 植物研究, 2010, 30(3): 369-372.
[6]	张　琳;祖元刚;牛卉颖;张颜滨;孙志强. 长春花(Catharanthus roseus*)中吲哚类生物碱含量的比较[J]. 植物研究, 2008, 28(2): 240-243.
[7]	马　伟;王振月*;陈立超;王宗权;赵海鹏;王　丹. 不同光质对毛脉酸模中蒽醌类成分的影响[J]. 植物研究, 2007, 27(6): 763-769.
[8]	郑春英;平文祥;刘松梅;祖元刚. HPLC-ELSD法在刺五加药材糖类成分分析中的应用[J]. 植物研究, 2007, 27(3): 376-379.
[9]	张艳华;孙立夫;施维林;王奇超;. HPLC法测定黑龙江省不同地区刺五加紫丁香苷的含量[J]. 植物研究, 2006, 26(1): 123-126.
[10]	付玉杰, 赵文灏, 侯春莲, 刘晓娜, 施晓光, 祖元刚. 超声提取-高效液相色谱法测定甘草中甘草酸含量[J]. 植物研究, 2005, 25(2): 210-212.
[11]	方芳, 李似姣, 管雁, 诸葛磊, 郭水良. 基于3种香茶菜属药用植物高效液相色谱的主成分分析[J]. 植物研究, 2004, 24(3): 351-356.

HPLC法测定甘薯叶片中的叶黄素

Determination of Lutein in Leaves of Sweet Potato by HPLC

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价