关键词: 斑茅, 鸟氨酸-&delta, -氨基转氨酶, 鸟氨酸途径, 定量PCR

Abstract: The complete cDNA sequence of orn-δ-aminotransferase (OAT) gene was obtained from Erianthus arundinaceus and was cloned by reverse-transcript-polymerase-chain-reaction (RT-PCR) and rapid amplification of cDNA end (RACE) technologies. The acquired gene was 1 680 bp in full length, encoding 454 amino acid residues. The amino acid sequence blast results showed that compared to that from mammal, higher plant and microorganism, δ-OAT gene from E.arundinaceus shared the highest homology (87%) with relative genera plant, Saccharum officinarum, 70% homology with other higher plants and 60% homology with animal. No N-terminal mitochondrial transit peptide (MTP) was found in the amino acid sequence encoded by δ-OAT gene from E.arundinaceus, which was the same as that from S.officinarum. Complete domain of OAT, rocD, was included in δ-OAT gene from E.arundinaceus. Expression level of δ-OAT gene from E.arundinaceus treated with 30% polyethylene glycol (PEG) was studied using real-time PCR technology, which showed that after treated 12 hours with PEG, the expression level reached the highest, 4.1 times as that of the comparison, but got lower after stressed 2 hours.

Key words: Erianthus arundinaceus, orn-&delta, -aminotransferase, orn pathway, real-time PCR