欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2009, Vol. 29 ›› Issue (3): 325-328.doi: 10.7525/j.issn.1673-5102.2009.03.014

• 论文 • 上一篇    下一篇

多年生黑麦草种子萌发及植株再生的研究

周小梅1;徐谨1;李君剑2*   

  1. (1.山西大学生命科学与技术学院,太原030006) (2.山西大学黄土高原研究所,太原030006)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 李君剑
  • 基金资助:
     

Study on the Germination and Plant Regeneration of Lolium perenne

ZHOU Xiao-Mei;XU Jin;LI Jun-Jian*   

  1. (1.Life Science and Technology College,Shanxi University,Taiyuan030006) (2.Institute of Loess Plateau,Shanxi University,Taiyuan030006)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: LI Jun-Jian
  • Supported by:
     

摘要: 对不同预处理条件下多年生黑麦草种子萌发、愈伤组织诱导以及植株再生进行了研究。结果表明,35℃恒温干燥处理3 h、浸泡16 h后,室温晾48 h的干湿交替及强度为1 000 GS磁场处理30 min均能使种子的发芽率得到显著提高。最适诱导培养基为MS+2,4-D 6 mg·L-1,有机添加物对于愈伤组织的生长影响并不显著,但可以改善其状态;较为适宜的分化培养基为MS+2 mg·L-1 6-BA或KT;生根培养基为附加0.5 mg·L-1 NAA的MS培养基。

关键词: 黑麦草, 种子, 预处理, 愈伤诱导, 植株再生

Abstract: We studied the effects of pre-treatments on seed germination, callus inducement and the plant regeneration of Lolium perenne. The results showed that pre-treatments including seeds drying 35℃ for 3 h, immersion for 16 hours then drying for 48 h and irradiation under 1 000 Gs magnetic field promoted the germination ratios significantly. MS medium was the optimal one in the three media tested. The 6 mg·L-1 2,4-D was suitable for inducing callus formation from seeds. The medium for callus differentiation into plantlets contained 2 mg·L-1 cytokinin (6-BA or KT). The basic MS medium adding 0.5 mg·L-1 NAA was best for regenerated plantlets rooting.

Key words: Lolium perenne, seeds, pre-treatments, callus, plant regeneration

中图分类号: