菊芋悬浮细胞对盐胁迫的生理响应

doi:10.7525/j.issn.1673-5102.2019.02.007

植物研究 ›› 2019, Vol. 39 ›› Issue (2): 222-228.doi: 10.7525/j.issn.1673-5102.2019.02.007

菊芋悬浮细胞对盐胁迫的生理响应

曹明武, 罗蕊, 安慧, 庞秋颖

东北林业大学盐碱地生物资源环境研究中心/东北盐碱植被恢复与重建教育部重点实验室, 哈尔滨 150040

收稿日期:2018-10-18 出版日期:2019-03-05 发布日期:2019-03-20
通讯作者: 庞秋颖 E-mail:qiuying@nefu.edu.cn
作者简介:曹明武(1994-),男,硕士研究生,研究方向为植物生理生态学。
基金资助:
国家自然科学基金（31470467）

Physiological Response of Suspension Cells of Helianthus tuberosus to NaCl Stress

CAO Ming-Wu, LUO Rui, AN Hui, PANG Qiu-Ying

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education/Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040

Received:2018-10-18 Online:2019-03-05 Published:2019-03-20
Supported by:
This work was supported by National Natural Science Foundation of China(31470467)

摘要/Abstract

摘要： 为探讨菊芋（Helianthus tuberosus）悬浮培养细胞对盐胁迫的生理响应，在0、50、100、150、200 mmol·L^-1NaCl处理下测定了细胞的生物量、相对细胞活力、抗氧化酶活性以及过氧化氢、丙二醛、脯氨酸、可溶性糖、可溶性蛋白质的含量，测定了总酚含量并鉴别、定量分析了14种酚类化合物。结果表明：盐胁迫显著减弱了菊芋悬浮细胞的活力，抑制了细胞的增长，200 mmol·L^-1NaCl处理下细胞生长基本上停止。盐胁迫诱发了细胞氧化胁迫，丙二醛含量显著增加，抗坏血酸过氧化物酶、过氧化物酶活性以及总酚含量、部分酚类化合物含量均随NaCl浓度升高而增加，酚类物质和抗氧化酶系统共同参与了应对氧化胁迫的抗氧化作用。脯氨酸在菊芋悬浮细胞应对NaCl渗透胁迫的渗透调节作用中扮演了重要的角色，而可溶性糖发挥的作用不大。

关键词: 菊芋, 悬浮细胞, 盐胁迫, 抗氧化作用, 渗透调节

Abstract: To investigate the physiological response of Helianthus tuberosus suspension cells to salt stress, biomass, viability, activity of antioxidant enzyme, the contents of hydrogen peroxide, malonaldehyde, proline, soluble sugar, soluble protein, total phenols were measured, and 14 phenolic compounds of suspension cells were analyzed under the treatments of 0, 50, 100, 150 and 200 mmol·L^-1 NaCl. The salt stress markedly weakened the H.tuberosus suspension cell viability, and the growth of suspension cells was obviously inhibited. Under the condition of 200 mmol·L^-1NaCl treatment, suspension cells almost stopped growing. Salt stress induced the oxidative stress, the content of malonaldehyde significantly increased, the activity of ascorbate peroxidase, peroxidase, the content of total phenols and part phenolic compounds increased with the increasing concentration of NaCl. Both phenolic compounds and antioxidant enzyme system participated in antioxidative process. Proline played an important role in the osmoregulation of H.tuberosus suspension cells responding to NaCl osmotic stress, meanwhile, the soluble sugar had little effect.

Key words: Helianthus tuberosus, suspension cells, salt stress, antioxidative process, osmoregulation

中图分类号:

Q945.78

曹明武, 罗蕊, 安慧, 庞秋颖. 菊芋悬浮细胞对盐胁迫的生理响应[J]. 植物研究, 2019, 39(2): 222-228.

CAO Ming-Wu, LUO Rui, AN Hui, PANG Qiu-Ying. Physiological Response of Suspension Cells of Helianthus tuberosus to NaCl Stress[J]. Bulletin of Botanical Research, 2019, 39(2): 222-228.

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菊芋悬浮细胞对盐胁迫的生理响应

Physiological Response of Suspension Cells of Helianthus tuberosus to NaCl Stress

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