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植物研究 ›› 2018, Vol. 38 ›› Issue (6): 852-859.doi: 10.7525/j.issn.1673-5102.2018.06.008

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白桦黄叶突变株叶色变化规律及苗高生长特性分析

任烁淇, 刘冰洋, 李雪莹, 邢建辉, 李政纶, 王楚, 冮慧欣, 刘桂丰, 姜静   

  1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040
  • 收稿日期:2018-06-29 出版日期:2018-11-15 发布日期:2018-11-01
  • 通讯作者: 姜静 E-mail:jiangjing1960@126.com
  • 作者简介:任烁淇(1997-),女,本科生,主要从事林木遗传育种研究。
  • 基金资助:
    大学生创新课题资助(课题号201710225167);国家自然基金项目资助(31570647)

Analysis of Leaf Color Variation and Height Growth Characteristics of Yellow-green Leaf Mutant in Birch

REN Shuo-Qi, LIU Bing-Yang, LI Xue-Ying, XING Jian-Hui, LI Zheng-Lun, WANG Chu, GANG Hui-Xin, LIU Gui-Feng, JIANG Jing   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2018-06-29 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    College Student Innovation Program(201710225167);National Natural Foundation of China(31570647)

摘要: 植物叶色突变体是研究植物光合作用机制、叶绿素生物合成分解途径的理想材料。为了研究白桦T-DNA突变株(yl)叶色与叶绿素含量的关系及yl苗高生长特性,实验以yl突变株为材料,测定其叶绿素与叶色的时序变化规律,分析生长特性。实验结果显示,白桦在生长季的不同发育时期,yl突变株的叶色一直呈现深黄绿色,色度计测定发现其叶片黄色程度及亮度均高于对照株系,叶色参数b*值分布于CIELab系统色拼图的黄色区域;从早春到9月中旬,yl突变株的叶绿素SPAD值一直显著低于对照株系(P<0.05),该值与a*值呈显著的负相关。yl突变株苗高显著低于对照株系,苗高年生长仅是对照株系均值的35.15%,其速生期内苗高日生长量均值(GD)也显著低于对照株系,是对照株系均值的58.50%,认为苗高生长量的降低是由于T-DNA插入突变影响了叶绿素生物合成的结果。

关键词: 白桦, 突变株, 叶色变异, 生长

Abstract: Leaf-color mutants are ideal materials for studying plant photosynthesis mechanisms and chlorophyll biosynthesis pathways. In order to study the relationship between the leaf color and the chlorophyll content, growth characteristics of the birch T-DNA mutant (yl), we measured the temporal variation of chlorophyll content, leaf color and the growth characteristics.The leaf color of the yl mutant displayed a dark yellow-green color during the different developmental stages. The yellowness and brightness of yl leaves were higher than that of the control line, and the parameter b* value of leaf color was distributed in the yellow area of the CIELab system color puzzle. The chlorophyll SPAD value of the yl mutant was significantly lower than that of the control lines (P<0.05) from early spring to mid-September. The height of the yl mutant was significantly lower than that of the control line, and the high annual growth rate was only 35.15% of the mean value of the control lines. The mean daily high growth (GD) was also significantly lower than control lines at fast growing stage, which was 58.50% of the control lines. Thus, it is considered that the decrease in high growth amount is due to the fact that the T-DNA insertion mutation affected the biosynthesis of chlorophyll.

Key words: birch, mutant, leaf color variation, growth

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