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植物研究 ›› 2018, Vol. 38 ›› Issue (3): 406-414.doi: 10.7525/j.issn.1673-5102.2018.03.013

• 研究报告 • 上一篇    下一篇

拟南芥中H2S与PLDα1响应干旱胁迫的作用研究

赵敏, 杨宁, 陈璐, 安炎黄, 李文领, 赵峰峰   

  1. 西北师范大学生命科学学院, 兰州 730070
  • 收稿日期:2017-12-12 出版日期:2018-05-15 发布日期:2018-05-17
  • 通讯作者: 杨宁,E-mail:xbsd-yn@163.com E-mail:xbsd-yn@163.com
  • 作者简介:赵敏(1993-),女,硕士研究生,主要从事植物细胞信号转导方面的研究。
  • 基金资助:
    国家自然科学基金(31660116)

Effects of H2S and PLDα1 on Drought Stress in Arabidopsis thaliana

ZHAO Min, YANG Ning, CHEN Lu, AN Yan-Huang, LI Wen-Ling, ZHAO Feng-Feng   

  1. College of Life Science, Northwest Normal University, Lanzhou 730070
  • Received:2017-12-12 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    National Natural Science Foundation of China(31660116)

摘要: 以野生型拟南芥WT、PLDα1合成缺陷突变体pldα1-1以及L-半胱氨酸脱巯基酶(L-cysteine desulfyrase,L-CDes)合成缺陷突变体lcd-4幼苗为材料,以0.3 mol·L-1甘露醇模拟干旱胁迫,研究磷脂酶Dα1(phospholipase Dα1,PLDα1)与气体信号分子硫化氢(hydrogen sulfide,H2S)响应干旱胁迫的作用及可能存在的信号关系。结果显示:干旱胁迫下,野生型拟南芥的H2S含量、PLD与L/D-CDes活性及其基因相对表达量均发生显著的变化。萌发率实验中,与WT相比,pldα1-1和lcd-4对干旱胁迫更加敏感,外施NaHS可以促进干旱胁迫下WT、pldα1-1以及lcd-4种子萌发及內源H2S产生,而外施PA能提高干旱胁迫下WT、pldα1-1种子萌发率及H2S含量,但对lcd-4萌发率及H2S含量没有显著影响。研究结果表明,信号分子H2S和PLDα1在拟南芥的干旱胁迫响应中发挥一定的作用,且H2S可能位于PLDα1的下游参与调控拟南芥种子萌发的信号过程。

关键词: 拟南芥, 干旱胁迫, 硫化氢, 磷脂酶Dα1

Abstract: The experiment was conducted to study the role of phospholipase Dα1(PLDα1) and the gas signal hydrogen sulfide(H2S) in response to drought stress and the possible signal relationship. The wild type Arabidopsis thaliana(WT), PLDα1 mutant(pldα1-1) and the LCD mutant plant(lcd-4) was used as experiment materials, and 0.3 mol·L-1 mannitol was used to simulate drought stress. The content of endogenous H2S, the activities of PLD, L/D-CDes, and the relative expression of PLDα1, LCD and DCD in wild type Arabidopsis were significantly changed under drought stress. Compared with WT, pldα1-1 and lcd-4 were more sensitive to drought condition. Application of NaHS could promote the seed germination and endogenous H2S production in WT, pldα1-1 and lcd-4. Exogenous addition of PA could increase the germination rate and endogenous H2S concentration in WT and pldα1-1, but had no significant effect in lcd-4. The signal molecules H2S and PLDα1 played important roles in the plant response to drought stress, and H2S might be located at downstream of PLDα1 in signal transduction process to regulate the seed germination of A. thaliana.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, phospholipase Dα1

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