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植物研究 ›› 2017, Vol. 37 ›› Issue (6): 855-860.doi: 10.7525/j.issn.1673-5102.2017.06.008

• 研究报告 • 上一篇    下一篇

Ca2+参与H2O2促进盐碱混合胁迫下燕麦种子的萌发和成苗

刘建新1,2, 刘秀丽1,2, 王金成1,2, 贾海燕1,2   

  1. 1. 甘肃省高校陇东生物资源保护与利用省级重点实验室, 庆阳 745000;
    2. 陇东学院生命科学与技术学院, 庆阳 745000
  • 收稿日期:2017-03-03 出版日期:2017-11-15 发布日期:2017-11-25
  • 作者简介:刘建新(1964-),男,教授,主要从事植物逆境生理方面的研究。
  • 基金资助:
    甘肃省庆阳市科技计划项目(KZ2014-19)资助

Effect of Ca2+ on Seed Germination and Seedling Formation of Oat Induced by H2O2 under Saline-Alkali Complex Stress

LIU Jian-Xin1,2, LIU Xiu-Li1,2, WANG Jin-Cheng1,2, JIA Hai-Yan1,2   

  1. 1. Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Qingyang 745000;
    2. College of Life Science and Technology, Longdong University, Qingyang 745000
  • Received:2017-03-03 Online:2017-11-15 Published:2017-11-25
  • Supported by:
    The project was financially supported by the science and technology plan project of Qingyang city in Gansu province(KZ2014-19)

摘要: 盐碱胁迫是制约作物高产优质的重要因素,Ca2+和H2O2作为信号分子参与作物逆境响应调节。为了解Ca2+是否参与H2O2对盐碱胁迫下植物种子萌发和成苗的调控,以燕麦(Avena nude)为试验材料,采用隶属函数分析方法,研究了胞外游离Ca2+螯合剂EGTA、质膜Ca2+通道阻断剂LaCl3和液泡膜Ca2+释放抑制剂钌红(RR)与H2O2共处理对盐碱混合(NaCl:Na2SO4:NaHCO3:Na2CO3=12:8:9:1)胁迫下种子萌发和成苗的影响。结果表明,25~200 mmol·L-1盐碱混合胁迫显著抑制燕麦的种子萌发和成苗,抑制程度随浓度提高而增强;0.001~2 mmol·L-1 H2O2能够促进燕麦种子的萌发和成苗,且0.5 mmol·L-1 H2O2可以显著缓解75 mmol·L-1盐碱混合胁迫对燕麦种子萌发和成苗的抑制作用;而EGTA、LaCl3和RR均能消减H2O2对盐碱混合胁迫下燕麦种子萌发和成苗的促进作用。表明Ca2+参与H2O2促进盐碱混合胁迫下燕麦种子萌发和成苗的信号转导过程。

关键词: 钙离子, 过氧化氢, 盐碱混合胁迫, 燕麦, 种子萌发和成苗

Abstract: Salinity-alkalinity stress is an important factor of restricting crop high-yield and good quality, Ca2+ and H2O2 as signal molecules involved in crop response to stress. In order to understand whether Ca2+ is involved in the regulation of H2O2 on the seed germination and seedling formation in plant under saline-alkali stress, using subordinate function analysis method, we studied the effects of extracellular dissociation Ca2+ chelant EGTA, plasma membrane Ca2+ channel blockers LaCl3, tonoplast Ca2+ release inhibitor ruthenium red(RR) and H2O2 on seed germination and seedling formation under saline-alkali complex stress(NaCl:Na2SO4:NaHCO3:Na2CO3=12:8:9:1) with oat(Avena nude). The results showed that 25-200 mmol·L-1 saline-alkali complex stress significantly inhibited seed germination and seedling formation of oat, and its inhibition was more obvious as the concentration was increased. While 0.001-2 mmol·L-1 H2O2 could promote the seed germination and seedling formation of oat, and 0.5 mmol·L-1 H2O2 significantly relieve the inhibition of 75 mmol·L-1 saline-alkali complex stress to seed germination and seedling formation of oat. After EGAT treatment, combining with LaCl3 and RR, the effect of H2O2 was decreased. Therefore, Ca2+ was involved in the signal transduction of seed germination and seedling formation of oat induced by H2O2 under saline-alkali mixed stress.

Key words: Ca2+, H2O2, saline-alkali mixed stress, oat, seed germination and seedling formation

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