长尖对齿藓原丝体快速培育的关键影响因子研究

doi:10.7525/j.issn.1673-5102.2017.02.005

植物研究 ›› 2017, Vol. 37 ›› Issue (2): 185-193.doi: 10.7525/j.issn.1673-5102.2017.02.005

长尖对齿藓原丝体快速培育的关键影响因子研究

赵洋^1,2, 李晓明⁴, 李茹雪³, 王春³, 卜崇峰^1,3

1. 中国科学院水利部水土保持研究所, 杨凌 712100;
2. 中国科学院大学, 北京 100049;
3. 西北农林科技大学水土保持研究所, 杨凌 712100;
4. 西北农林科技大学资源与环境学院, 杨凌 712100

收稿日期:2016-11-03 出版日期:2017-03-15 发布日期:2017-03-11
通讯作者: 卜崇峰,E-mail:buchongfeng@163.com E-mail:buchongfeng@163.com
作者简介:赵洋(1992-),女,硕士研究生,主要从事苔藓结皮原丝体的快速培育研究。
基金资助:
中国科学院西部之光（2014-91）；中央高校优秀青年科研业务专项基金（2014YQ006）；国家自然科学基金（41541008）

The Research of Key Influence Factors of Rapid Culture of the Protonema of Didymodon ditrichoides

ZHAO Yang^1,2, LI Xiao-Ming⁴, LI Ru-Xue³, WANG Chun³, BU Chong-Feng^1,3

1. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100;
2. University of Chinese Academy of Sciences, Beijing 100049;
3. Institute of Soil and Water Conservation, Northwest A & F University, Yangling 712100;
4. College of Nature Resource and Environment, Northwest A & F University, Yangling 712100

Received:2016-11-03 Online:2017-03-15 Published:2017-03-11
Supported by:
The Light of West China of Chinese Academy of Sciences(2014-91);The Fundamental Research Funds for the Central Universities (2014YQ006);The National Nature Science Foundation of China(41541008)

摘要/Abstract

摘要： 为了给苔藓结皮野外恢复的大规模接种提供丰富种源，本文利用0.1% NaClO（10、15、20、30、45、60、120 s）和0.1% HgCl₂（10、15、20、30、45、60、120 s）两种消毒液，采用Knop、MS和Hoagland 3种培养基，设置5.5、6、6.5、7、7.5、8、8.5七个水平的pH值，通过单因素试验设计方法，测定长尖对齿藓[Didymodon ditrichoides（Broth.）]茎叶体的成活率、原丝体长度和分枝数等指标，探讨影响长尖对齿藓原丝体扩繁的关键因子。结果表明：（1）消毒方式、培养基及pH均对长尖对齿藓茎叶体的成活率、原丝体长度和分枝数有显著影响（P<0.05），影响大小依次均为消毒方式 > 培养基 > pH；（2）最适合的消毒方式为：0.1% NaClO消毒15~20 s；（3）最适合长尖对齿藓原丝体生长的培养基是Knop和Hoagland培养基；（4）最适合长尖对齿藓原丝体生长的pH是7.5。从而得出快速培育长尖对齿藓原丝体的最佳组合为：0.1% NaClO消毒15~20 s+Hoagland/Knop+pH=7.5。本文的研究结果可以缩短长尖对齿藓生长周期以进行快速繁殖，为苔藓结皮的快速培育以及工程化应用提供一定的借鉴。

关键词: 长尖对齿藓, 原丝体, 消毒方式, 培养基, pH

Abstract: In order to provide a rich source of mass vaccination for the field restoration of moss crust, we used two kinds of disinfection method, 0.1% NaClO(10, 15, 20, 30, 45, 60, 120 s) and 0.1% HgCl₂(10, 15, 20, 30, 45, 60, 120 s), with Knop, MS and Hoagland as culture media, and set the pH values(5.5, 6, 6.5, 7, 7.5, 8, 8.5), through the single factor experiment design method, we determined the survival rate of the cormus of[Didymodon ditrichoides(Broth.)], protonema length and number of branches, thereby exploring key factors that affect the propagation of the protonema of D.ditrichoides. The results are as follows:(1) The disinfection method, culture medium and pH value have significant influence upon the survival rate of the cormus of D.ditrichoides, length of protonema and number of branches(P<0.05), in a descending order of disinfection method, culture medium, and pH value; (2) The most suitable disinfection method is 0.1% NaClO with a duration of 15-20 s; (3) The most suitable culture medium for the growth of the protonema of D.ditrichoides is Knop and Hoagland; (4) The most suitable pH value for the growth of protonema of D.ditrichoides is 7.5. Therefore, the best combination of factors for rapid cultivation of the protonema of D.ditrichoides is 0.1% NaClO disinfection 15-20 s+Hoagland/Knop+pH=7.5. Our experiment can be determined to shorten the growth cycle of D.ditrichoides and realize rapid propagation, thereby providing experience for the rapid culture and engineering application of moss crust.

Key words: Didymodon ditrichoides, protonema, disinfection method, culture medium, pH

中图分类号:

S154

赵洋, 李晓明, 李茹雪, 王春, 卜崇峰. 长尖对齿藓原丝体快速培育的关键影响因子研究[J]. 植物研究, 2017, 37(2): 185-193.

ZHAO Yang, LI Xiao-Ming, LI Ru-Xue, WANG Chun, BU Chong-Feng. The Research of Key Influence Factors of Rapid Culture of the Protonema of Didymodon ditrichoides[J]. Bulletin of Botanical Research, 2017, 37(2): 185-193.

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长尖对齿藓原丝体快速培育的关键影响因子研究

The Research of Key Influence Factors of Rapid Culture of the Protonema of Didymodon ditrichoides

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