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植物研究 ›› 2009, Vol. 29 ›› Issue (4): 505-508.doi: 10.7525/j.issn.1673-5102.2009.04.021

• 论文 • 上一篇    下一篇

刺五加组培快繁的研究

褚丽敏;孙周平*   

  1. (沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳110161)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-20 发布日期:2009-07-20
  • 通讯作者: 孙周平
  • 基金资助:
     

Tissue Culture and Rapid Propagation of Acanthopanax senticosus

CHU Li-Min;SUN Zhou-Ping*   

  1. (Key Laboratory of Protected Horticulture of Liaoning Province,College of Horticulture,Shenyang Agricultural University,Shenyang110161)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: SUN Zhou-Ping
  • Supported by:
     

摘要: 以刺五加腋芽为外植体,通过比较不同的基本培养基(WPM、MS、1/2MS、White)和植物生长调节物质(6-BA、NAA、IAA、IBA)的组合对腋芽诱导、增殖及生根的影响,来建立一套高效的离体芽再生体系。试验结果表明,最佳芽再生培养基为WPM+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1,芽萌发率可达90%;最佳芽增殖培养基为WPM+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1,增殖倍数为4.8;最佳生根培养基为White+IBA 0.5 mg·L-1+IAA 1.5 mg·L-1,生根率可达85.2%。

关键词: 刺五加, 腋芽, 组织培养, 快速繁殖

Abstract: Using axillary bud explants,an efficient plant regeneration system via organogenesis was established for Acanthopanax senticosus.The results showed that the optimum regeneration medium was WPM+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1,germination rate was 90%;the propagating medium was WPM+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1, propagating times was 4.8; the rooting medium was White+IBA 0.5 mg·L-1+IAA 1.5 mg·L-1,the rooting rate was 85.2%.

Key words: Acanthopanax senticosus, axillary bud, tissue culture, rapid propagation

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