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植物研究 ›› 2009, Vol. 29 ›› Issue (2): 187-192.doi: 10.7525/j.issn.1673-5102.2009.02.011

• 论文 • 上一篇    下一篇

濒危植物珙桐的组织培养与植株再生

邹利娟1,2;苏智先2*;胡进耀2;吴庆贵2;苏瑞军2;余阿梅1,2   

  1. (1.西华师范大学环境科学与生物多样性保护省级重点实验室,南充637002) (2.四川省生态与环境技术重点实验室,绵阳 621000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 苏智先
  • 基金资助:
     

Tissue Culture and Plant Regeneration of Endangered Plant Davidia involucrate Baill

ZOU Li-Juan;SU Zhi-Xian*;HU Jin-Yao;WU Qing-Gui;SU Rui-Jun;YU A-Mei;   

  1. (1.Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation,China West Normal University,Nanchong 637002) (2.Sichuan Provincial Key Laboratory of Ecology and Environmental Technology,Mianyang 621000)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: SU Zhi-Xian
  • Supported by:
     

摘要: 以珙桐冬芽为材料进行组织培养和植株再生研究,结果表明:珙桐冬芽直接诱导丛生芽的最适培养基为WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1;珙桐带芽茎段增殖的适宜培养基为WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1;生根最佳培养基为White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1,在此条件下,根发育良好,植株健壮;组培苗炼苗后移栽,成活率可达80%。

关键词: 珙桐, 冬芽, 丛生芽, 茎段增殖, 植株再生

Abstract: Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated: the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM+NAA 0.2 mg·L-1+6-BA 3.0 mg·L-1+AC 2.0 g·L-1; the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM+NAA 0.05 mg·L-1+6-BA 1.0 mg·L-1+GA3 2.0 mg·L-1+AC 2.0 g·L-1; the medium for rooting culture was White+IBA3.0 mg·L-1+6-BA 1.0 mg·L-1+AC 2.0 g·L-1, under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation.

Key words: Davidia involucrate Baill, winter bud, clump shoot, shoot segment multiplying, plant regeneration

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